Literature DB >> 19098221

Cytosine deaminase as a negative selection marker for gene disruption and replacement in the genus Streptomyces and other actinobacteria.

Marie-Pierre Dubeau1, Mariana Gabriela Ghinet, Pierre-Etienne Jacques, Nancy Clermont, Carole Beaulieu, Ryszard Brzezinski.   

Abstract

We developed a novel negative selection system for actinobacteria based on cytosine deaminase (CodA). We constructed vectors that include a synthetic gene encoding the CodA protein from Escherichia coli optimized for expression in Streptomyces species. Gene disruption and the introduction of an unmarked in-frame deletion were successfully achieved with these vectors.

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Year:  2008        PMID： 19098221      PMCID： PMC2643560          DOI： 10.1128/AEM.02139-08

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  22 in total

1.  An improved aminoglycoside resistance gene cassette for use in gram-negative bacteria and Streptomyces.

Authors:  F Denis; R Brzezinski
Journal:  FEMS Microbiol Lett       Date:  1991-07-01       Impact factor: 2.742

2.  Transfer of the bacterial gene for cytosine deaminase to mammalian cells confers lethal sensitivity to 5-fluorocytosine: a negative selection system.

Authors:  C A Mullen; M Kilstrup; R M Blaese
Journal:  Proc Natl Acad Sci U S A       Date:  1992-01-01       Impact factor: 11.205

3.  Antibiotic resistance gene cassettes derived from the omega interposon for use in E. coli and Streptomyces.

Authors:  M H Blondelet-Rouault; J Weiser; A Lebrihi; P Branny; J L Pernodet
Journal:  Gene       Date:  1997-05-06       Impact factor: 3.688

4.  Cytosine deaminase MX cassettes as positive/negative selectable markers in Saccharomyces cerevisiae.

Authors:  Phillip E Hartzog; Bradly P Nicholson; John H McCusker
Journal:  Yeast       Date:  2005-07-30       Impact factor: 3.239

5.  Determining the functionality of putative Tat-dependent signal peptides in Streptomyces coelicolor A3(2) by using two different reporter proteins.

Authors:  Haiming Li; Pierre-Étienne Jacques; Mariana Gabriela Ghinet; Ryszard Brzezinski; Rolf Morosoli
Journal:  Microbiology (Reading)       Date:  2005-07       Impact factor: 2.777

6.  LITMUS: multipurpose cloning vectors with a novel system for bidirectional in vitro transcription.

Authors:  P D Evans; S N Cook; P D Riggs; C J Noren
Journal:  Biotechniques       Date:  1995-07       Impact factor: 1.993

7.  Evolutionary relationships between sugar kinases and transcriptional repressors in bacteria.

Authors:  F Titgemeyer; J Reizer; A Reizer; M H Saier
Journal:  Microbiology (Reading)       Date:  1994-09       Impact factor: 2.777

8.  Use of rpsL for dominance selection and gene replacement in Streptomyces roseosporus.

Authors:  T J Hosted; R H Baltz
Journal:  J Bacteriol       Date:  1997-01       Impact factor: 3.490

9.  Pathways of pyrimidine salvage in Streptomyces.

Authors:  Lee E Hughes; Debrah A Beck; Gerard A O'Donovan
Journal:  Curr Microbiol       Date:  2004-12-08       Impact factor: 2.188

10.  JCat: a novel tool to adapt codon usage of a target gene to its potential expression host.

Authors:  Andreas Grote; Karsten Hiller; Maurice Scheer; Richard Münch; Bernd Nörtemann; Dietmar C Hempel; Dieter Jahn
Journal:  Nucleic Acids Res       Date:  2005-07-01       Impact factor: 16.971
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  18 in total

1.  NMR line shape analysis of a multi-state ligand binding mechanism in chitosanase.

Authors:  Shoko Shinya; Mariana G Ghinet; Ryszard Brzezinski; Kyoko Furuita; Chojiro Kojima; Sneha Shah; Evgenii L Kovrigin; Tamo Fukamizo
Journal:  J Biomol NMR       Date:  2017-04-09       Impact factor: 2.835

2.  Properties of CsnR, the transcriptional repressor of the chitosanase gene, csnA, of Streptomyces lividans.

Authors:  Marie-Pierre Dubeau; Dominic Poulin-Laprade; Mariana Gabriela Ghinet; Ryszard Brzezinski
Journal:  J Bacteriol       Date:  2011-03-25       Impact factor: 3.490

3.  Beta-glucuronidase as a sensitive and versatile reporter in actinomycetes.

Authors:  Maksym Myronovskyi; Elisabeth Welle; Viktor Fedorenko; Andriy Luzhetskyy
Journal:  Appl Environ Microbiol       Date:  2011-06-17       Impact factor: 4.792

4.  A bacterial gene codA encoding cytosine deaminase is an effective conditional negative selectable marker in Glycine max.

Authors:  Min Shao; Jean-Michel Michno; Sara K Hotton; Ann Blechl; James Thomson
Journal:  Plant Cell Rep       Date:  2015-06-17       Impact factor: 4.570

5.  Markerless Gene Deletion with Cytosine Deaminase in Thermus thermophilus Strain HB27.

Authors:  Lei Wang; Jana Hoffmann; Hildegard Watzlawick; Josef Altenbuchner
Journal:  Appl Environ Microbiol       Date:  2015-12-11       Impact factor: 4.792

Review 6.  Engineering microbial hosts for production of bacterial natural products.

Authors:  Mingzi M Zhang; Yajie Wang; Ee Lui Ang; Huimin Zhao
Journal:  Nat Prod Rep       Date:  2016-04-13       Impact factor: 13.423

7.  Semisynthesis of an Anticancer DPAGT1 Inhibitor from a Muraymycin Biosynthetic Intermediate.

Authors:  Katsuhiko Mitachi; Shou M Kurosu; Shakiba Eslamimehr; Maddie R Lemieux; Yoshimasa Ishizaki; William M Clemons; Michio Kurosu
Journal:  Org Lett       Date:  2019-01-30       Impact factor: 6.005

8.  Modification of genetic regulation of a heterologous chitosanase gene in Streptomyces lividans TK24 leads to chitosanase production in the absence of chitosan.

Authors:  Marie-Pierre Dubeau; Isabelle Guay; Ryszard Brzezinski
Journal:  Microb Cell Fact       Date:  2011-02-10       Impact factor: 5.328

9.  Cytosine deaminase as a negative selectable marker for the microalgal chloroplast: a strategy for the isolation of nuclear mutations that affect chloroplast gene expression.

Authors:  Rosanna E B Young; Saul Purton
Journal:  Plant J       Date:  2014-10-25       Impact factor: 6.417

10.  Improving acarbose production and eliminating the by-product component C with an efficient genetic manipulation system of Actinoplanes sp. SE50/110.

Authors:  Qinqin Zhao; Huixin Xie; Yao Peng; Xinran Wang; Linquan Bai
Journal:  Synth Syst Biotechnol       Date:  2017-11-27
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