Literature DB >> 9197550

Antibiotic resistance gene cassettes derived from the omega interposon for use in E. coli and Streptomyces.

M H Blondelet-Rouault1, J Weiser, A Lebrihi, P Branny, J L Pernodet.   

Abstract

Three antibiotic resistance gene cassettes, derived from the omega interposon (Prentki and Krisch (1984) Gene 29, 303-313) were constructed. These cassettes carry different antibiotic resistance genes, conferring resistance to geneticin, hygromycin or viomycin, flanked by short inverted repeats containing transcription and translation termination signals and synthetic polylinkers. These cassettes were designated omega aac, omega hyg and omega vph. Resistance phenotypes conferred by these constructions are selectable in E. coli and Streptomyces. These cassettes can be used for insertional mutagenesis or for vector construction.

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Year:  1997        PMID： 9197550     DOI： 10.1016/s0378-1119(97)00014-0

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  69 in total

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3.  Rapid and sequential movement of individual chromosomal loci to specific subcellular locations during bacterial DNA replication.

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4.  Function of the cytochrome bc1-aa3 branch of the respiratory network in mycobacteria and network adaptation occurring in response to its disruption.

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Journal:  J Bacteriol       Date:  2005-09       Impact factor: 3.490

5.  The dasABC gene cluster, adjacent to dasR, encodes a novel ABC transporter for the uptake of N,N'-diacetylchitobiose in Streptomyces coelicolor A3(2).

Authors:  Akihiro Saito; Tomonori Shinya; Katsushiro Miyamoto; Tomofumi Yokoyama; Hanae Kaku; Eiichi Minami; Naoto Shibuya; Hiroshi Tsujibo; Yoshiho Nagata; Akikazu Ando; Takeshi Fujii; Kiyotaka Miyashita
Journal:  Appl Environ Microbiol       Date:  2007-03-09       Impact factor: 4.792

6.  Effect of superoxide dismutase gene inactivation on virulence of Pseudomonas aeruginosa PAO1 toward the silkworm, Bombyx mori.

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Journal:  Appl Environ Microbiol       Date:  2007-01-12       Impact factor: 4.792

7.  Dynamics and control of biofilms of the oligotrophic bacterium Caulobacter crescentus.

Authors:  Plamena Entcheva-Dimitrov; Alfred M Spormann
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8.  The leucine-responsive regulatory protein, Lrp, activates transcription of the fim operon in Salmonella enterica serovar typhimurium via the fimZ regulatory gene.

Authors:  Kirsty A McFarland; Sacha Lucchini; Jay C D Hinton; Charles J Dorman
Journal:  J Bacteriol       Date:  2007-11-02       Impact factor: 3.490

9.  The lysis system of the Streptomyces aureofaciens phage mu1/6.

Authors:  Jarmila Farkasovská; Andrej Godány
Journal:  Curr Microbiol       Date:  2008-09-25       Impact factor: 2.188

10.  Replicase, excisionase, and integrase genes of the Streptomyces element pSAM2 constitute an operon positively regulated by the pra gene.

Authors:  G Sezonov; A M Duchêne; A Friedmann; M Guérineau; J L Pernodet
Journal:  J Bacteriol       Date:  1998-06       Impact factor: 3.490
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