Literature DB >> 1909480

Purification and characterization of human immunodeficiency virus type 1 nef gene product expressed by a recombinant baculovirus.

Y Matsuura1, M Maekawa, S Hattori, N Ikegami, A Hayashi, S Yamazaki, C Morita, Y Takebe.   

Abstract

We have constructed the recombinant baculovirus which expresses the human immunodeficiency virus type 1 negative factor (nef) gene. Spodoptera frugiperda cells infected with the recombinant virus produced a 27-kDa protein which reacted with rabbit antisera raised against a carboxy-terminal synthetic peptide of the Nef protein by immunoblot analysis. Labeling experiment showed that the recombinant Nef protein was myristoylated. The recombinant Nef protein was purified to near homogeneity by DEAE-Sephacel, phenyl-Sepharose 4B, blue-Sepharose, and Sephadex G-150 column chromatography. No detectable GTP binding activity was observed in the purified recombinant Nef product.

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Year:  1991        PMID： 1909480     DOI： 10.1016/0042-6822(91)90428-e

Source DB:  PubMed          Journal:  Virology        ISSN： 0042-6822            Impact factor:   3.616


  9 in total

1.  Repair and evolution of nef in vivo modulates simian immunodeficiency virus virulence.

Authors:  A M Whatmore; N Cook; G A Hall; S Sharpe; E W Rud; M P Cranage
Journal:  J Virol       Date:  1995-08       Impact factor: 5.103

2.  Structure, dynamics, and Hck interaction of full-length HIV-1 Nef.

Authors:  Jinwon Jung; In-Ja L Byeon; Jinwoo Ahn; Angela M Gronenborn
Journal:  Proteins       Date:  2011-03-01

3.  Biological characterization of human immunodeficiency virus type 1 and type 2 mutants in human peripheral blood mononuclear cells.

Authors:  H Akari; J Sakuragi; Y Takebe; K Tomonaga; M Kawamura; M Fukasawa; T Miura; T Shinjo; M Hayami
Journal:  Arch Virol       Date:  1992       Impact factor: 2.574

4.  Mutational analysis of the human immunodeficiency virus type 1 Eli Nef function.

Authors:  E Zazopoulos; W A Haseltine
Journal:  Proc Natl Acad Sci U S A       Date:  1992-07-15       Impact factor: 11.205

5.  Human immunodeficiency virus type 1 Nef-induced down-modulation of CD4 is due to rapid internalization and degradation of surface CD4.

Authors:  S S Rhee; J W Marsh
Journal:  J Virol       Date:  1994-08       Impact factor: 5.103

6.  Human immunodeficiency virus type 1 Nef associates with a cellular serine kinase in T lymphocytes.

Authors:  E T Sawai; A Baur; H Struble; B M Peterlin; J A Levy; C Cheng-Mayer
Journal:  Proc Natl Acad Sci U S A       Date:  1994-02-15       Impact factor: 11.205

7.  Expression of the HIV-1 Nef protein in the baculovirus system: investigation of anti-Nef antibodies response in human sera and subcellular localization of Nef.

Authors:  N Kienzle; M Enders; M Buck; H Siakkou; S Jahn; G Petzold; K E Schneweis; M Bachmann; W E Müller; N Müller-Lantzsch
Journal:  Arch Virol       Date:  1992       Impact factor: 2.574

Review 8.  The role of unintegrated DNA in HIV infection.

Authors:  Richard D Sloan; Mark A Wainberg
Journal:  Retrovirology       Date:  2011-07-01       Impact factor: 4.602

9.  The human immunodeficiency virus-1 nef gene product: a positive factor for viral infection and replication in primary lymphocytes and macrophages.

Authors:  M D Miller; M T Warmerdam; I Gaston; W C Greene; M B Feinberg
Journal:  J Exp Med       Date:  1994-01-01       Impact factor: 14.307
  9 in total

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