Literature DB >> 1908476

Determination of human urinary hyaluronic acid, chondroitin sulphate and dermatan sulphate as their unsaturated disaccharides by high-performance liquid chromatography.

H Toyoda1, K Motoki, M Tanikawa, K Shinomiya, H Akiyama, T Imanari.   

Abstract

A method for the determination of hyaluronic acid (HA), chondroitin sulphate (CS) and dermatan sulphate (DS) was developed. HA, CS and DS were converted to the corresponding unsaturated disaccharides by digestion with chondroitinase ABC and/or chondroitinase AC-II and determined by high-performance liquid chromatography with fluorimetric detection using 2-cyanoacetamide as a post-column derivatization reagent. The calibration graphs for the unsaturated disaccharides were linear over the range 2 ng - 2 micrograms for each unsaturated disaccharide. This method was applied to the analysis of normal human urine.

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Year:  1991        PMID： 1908476     DOI： 10.1016/0378-4347(91)80378-p

Source DB:  PubMed          Journal:  J Chromatogr


  8 in total

1.  Hyaluronan is not elevated in urine or serum in Hutchinson-Gilford Progeria Syndrome.

Authors:  Leslie B Gordon; Ingrid A Harten; Anthony Calabro; Geetha Sugumaran; Antonei B Csoka; W Ted Brown; Vincent Hascall; Bryan P Toole
Journal:  Hum Genet       Date:  2003-05-01       Impact factor: 4.132

2.  Analysis of glycosaminoglycans in rabbit cornea after excimer laser keratectomy.

Authors:  T Kato; K Nakayasu; K Ikegami; T Obara; T Kanayama; A Kanai
Journal:  Br J Ophthalmol       Date:  1999-05       Impact factor: 4.638

3.  Effect of 6-O-sulfonate hexosamine residue on anticoagulant activity of fully O-sulfonated glycosaminoglycans.

Authors:  T Toida; A Suzuki; K Nakajima; A Chaidedgumjorn; T Imanari
Journal:  Glycoconj J       Date:  2000-06       Impact factor: 2.916

Review 4.  Biosynthesis of glycosaminoglycans: associated disorders and biochemical tests.

Authors:  Florin Sasarman; Catalina Maftei; Philippe M Campeau; Catherine Brunel-Guitton; Grant A Mitchell; Pierre Allard
Journal:  J Inherit Metab Dis       Date:  2015-12-21       Impact factor: 4.982

Review 5.  Glycosaminoglycan glycomics using mass spectrometry.

Authors:  Joseph Zaia
Journal:  Mol Cell Proteomics       Date:  2013-01-16       Impact factor: 5.911

Review 6.  On-line separations combined with MS for analysis of glycosaminoglycans.

Authors:  Joseph Zaia
Journal:  Mass Spectrom Rev       Date:  2009 Mar-Apr       Impact factor: 10.946

7.  Chondroitin sulphate structure affects its immunological activities on murine splenocytes sensitized with ovalbumin.

Authors:  Hiroshi Akiyama; Shinobu Sakai; Robert J Linhardt; Yukihiro Goda; Toshihiko Toida; Tamio Maitani
Journal:  Biochem J       Date:  2004-08-15       Impact factor: 3.857

8.  A chip-based amide-HILIC LC/MS platform for glycosaminoglycan glycomics profiling.

Authors:  Gregory O Staples; Michael J Bowman; Catherine E Costello; Alicia M Hitchcock; James M Lau; Nancy Leymarie; Christine Miller; Hicham Naimy; Xiaofeng Shi; Joseph Zaia
Journal:  Proteomics       Date:  2009-02       Impact factor: 3.984
  8 in total

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