Literature DB >> 19083998

Unequal representation of different unique genomic DNA sequences in the cell-free plasma DNA of individual donors.

William M Puszyk1, Francesco Crea, Robert W Old.   

Abstract

OBJECTIVES: To assess whether different genomic cell-free DNAs are equally abundant in the plasma of individual donors, and any relationship between DNA methylation and representation in plasma. DESIGN AND METHODS: The concentrations of DNA in plasma were determined by real-time PCR.
RESULTS: Different DNA sequences were not equally represented. The relative abundances were similar in different donors.
CONCLUSIONS: Different DNA sequences are not equally abundant in plasma, with no relationship between DNA methylation and abundance.

Mesh:

Substances:

Year:  2008        PMID: 19083998     DOI: 10.1016/j.clinbiochem.2008.11.006

Source DB:  PubMed          Journal:  Clin Biochem        ISSN: 0009-9120            Impact factor:   3.281


  6 in total

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6.  Direct quantification of cell-free, circulating DNA from unpurified plasma.

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  6 in total

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