Literature DB >> 19083807

2006 GEP-ISFG collaborative exercise on mtDNA: reflections about interpretation, artefacts, and DNA mixtures.

L Prieto1, A Alonso, C Alves, M Crespillo, M Montesino, A Picornell, A Brehm, J L Ramírez, M R Whittle, M J Anjos, I Boschi, J Buj, M Cerezo, S Cardoso, R Cicarelli, D Comas, D Corach, C Doutremepuich, R M Espinheira, I Fernández-Fernández, S Filippini, Julia Garcia-Hirschfeld, A González, B Heinrichs, A Hernández, F P N Leite, R P Lizarazo, A M López-Parra, M López-Soto, J A Lorente, B Mechoso, I Navarro, S Pagano, J J Pestano, J Puente, E Raimondi, A Rodríguez-Quesada, M F Terra-Pinheiro, L Vidal-Rioja, C Vullo, A Salas.   

Abstract

We report the results of the seventh edition of the GEP-ISFG mitochondrial DNA (mtDNA) collaborative exercise. The samples submitted to the participant laboratories were blood stains from a maternity case and simulated forensic samples, including a case of mixture. The success rate for the blood stains was moderate ( approximately 77%); even though four inexperienced laboratories concentrated about one-third of the total errors. A similar success was obtained for the analysis of mixed samples (78.8% for a hair-saliva mixture and 69.2% for a saliva-saliva mixture). Two laboratories also dissected the haplotypes contributing to the saliva-saliva mixture. Most of the errors were due to reading problems and misinterpretation of electropherograms, demonstrating once more that the lack of a solid devised experimental approach is the main cause of error in mtDNA testing.

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Year:  2007        PMID: 19083807     DOI: 10.1016/j.fsigen.2007.10.010

Source DB:  PubMed          Journal:  Forensic Sci Int Genet        ISSN: 1872-4973            Impact factor:   4.882


  4 in total

Review 1.  Separation/extraction, detection, and interpretation of DNA mixtures in forensic science (review).

Authors:  Ruiyang Tao; Shouyu Wang; Jiashuo Zhang; Jingyi Zhang; Zihao Yang; Xiang Sheng; Yiping Hou; Suhua Zhang; Chengtao Li
Journal:  Int J Legal Med       Date:  2018-05-25       Impact factor: 2.686

2.  Detecting heteroplasmy from high-throughput sequencing of complete human mitochondrial DNA genomes.

Authors:  Mingkun Li; Anna Schönberg; Michael Schaefer; Roland Schroeder; Ivane Nasidze; Mark Stoneking
Journal:  Am J Hum Genet       Date:  2010-08-13       Impact factor: 11.025

3.  Results of a collaborative study on DNA identification of aged bone samples.

Authors:  Daniel Vanek; Bruce Budowle; Jitka Dubska-Votrubova; Angie Ambers; Jan Frolik; Martin Pospisek; Ahmed Anwar Al Afeefi; Khalid Ismaeil Al Hosani; Marie Allen; Khudooma Saeed Al Naimi; Dina Al Salafi; Wafa Ali Rashid Al Tayyari; Wendy Arguetaa; Michel Bottinelli; Magdalena M Bus; Jan Cemper-Kiesslich; Olivier Cepil; Greet De Cock; Stijn Desmyter; Hamid El Amri; Hicham El Ossmani; Ruth Galdies; Sebastian Grün; Francois Guidet; Anna Hoefges; Cristian Bogdan Iancu; Petra Lotz; Alessandro Maresca; Marion Nagy; Jindrich Novotny; Hajar Rachid; Jessica Rothe; Marguerethe Stenersen; Mishel Stephenson; Alain Stevanovitch; Juliane Strien; Denilce R Sumita; Joanna Vella; Judith Zander
Journal:  Croat Med J       Date:  2017-06-14       Impact factor: 1.351

4.  Mitochondrial and nuclear disease panel (Mito-aND-Panel): Combined sequencing of mitochondrial and nuclear DNA by a cost-effective and sensitive NGS-based method.

Authors:  Angela Abicht; Florentine Scharf; Stephanie Kleinle; Ulrike Schön; Elke Holinski-Feder; Rita Horvath; Anna Benet-Pagès; Isabel Diebold
Journal:  Mol Genet Genomic Med       Date:  2018-11-08       Impact factor: 2.183

  4 in total

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