| Literature DB >> 19083807 |
L Prieto1, A Alonso, C Alves, M Crespillo, M Montesino, A Picornell, A Brehm, J L Ramírez, M R Whittle, M J Anjos, I Boschi, J Buj, M Cerezo, S Cardoso, R Cicarelli, D Comas, D Corach, C Doutremepuich, R M Espinheira, I Fernández-Fernández, S Filippini, Julia Garcia-Hirschfeld, A González, B Heinrichs, A Hernández, F P N Leite, R P Lizarazo, A M López-Parra, M López-Soto, J A Lorente, B Mechoso, I Navarro, S Pagano, J J Pestano, J Puente, E Raimondi, A Rodríguez-Quesada, M F Terra-Pinheiro, L Vidal-Rioja, C Vullo, A Salas.
Abstract
We report the results of the seventh edition of the GEP-ISFG mitochondrial DNA (mtDNA) collaborative exercise. The samples submitted to the participant laboratories were blood stains from a maternity case and simulated forensic samples, including a case of mixture. The success rate for the blood stains was moderate ( approximately 77%); even though four inexperienced laboratories concentrated about one-third of the total errors. A similar success was obtained for the analysis of mixed samples (78.8% for a hair-saliva mixture and 69.2% for a saliva-saliva mixture). Two laboratories also dissected the haplotypes contributing to the saliva-saliva mixture. Most of the errors were due to reading problems and misinterpretation of electropherograms, demonstrating once more that the lack of a solid devised experimental approach is the main cause of error in mtDNA testing.Entities:
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Year: 2007 PMID: 19083807 DOI: 10.1016/j.fsigen.2007.10.010
Source DB: PubMed Journal: Forensic Sci Int Genet ISSN: 1872-4973 Impact factor: 4.882