Literature DB >> 19082490

Functional reconstitution of RLIP76 catalyzing ATP-dependent transport of glutathione-conjugates.

Sharad S Singhal1, Archana Sehrawat, Amee Mehta, Mukesh Sahu, Sanjay Awasthi.   

Abstract

RLIP76, a stress-responsive, multi-functional protein with multi-specific transport activity towards glutathione-conjugates (GS-E) and chemotherapeutic agents is frequently overexpressed in malignant cells. Our recent studies suggest that it plays a prominent anti-apoptotic role selectively in cancer cells. The present studies were performed to compare RLIP76 activity towards glutathione-conjugates in recombinant and K562 human erythroleukemia cells. The purity and identity of recombinant and K562 RLIP76 was established by SDS-PAGE and Western blot analysis. These studies confirmed the origin of the 38 kDa protein, previously referred to as DNP-SG ATPase, from RLIP76. Comparison of ATPase activity and transport kinetics for DNP-SG and GS-HNE between recombinant vs. K562 RLIP76 revealed higher specific activity of ATPase and transport for recombinant purified RLIP76, indicating that additional factors present in recombinant purified RLIP76 can modulate its transport activity.

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Year:  2009        PMID： 19082490      PMCID： PMC2664085     

Source DB:  PubMed          Journal:  Int J Oncol        ISSN： 1019-6439            Impact factor:   5.650


  29 in total

1.  RLIP76 is the major ATP-dependent transporter of glutathione-conjugates and doxorubicin in human erythrocytes.

Authors:  R Sharma; S S Singhal; J Cheng; Y Yang; A Sharma; P Zimniak; S Awasthi; Y C Awasthi
Journal:  Arch Biochem Biophys       Date:  2001-07-15       Impact factor: 4.013

2.  Epsin binds to the EH domain of POB1 and regulates receptor-mediated endocytosis.

Authors:  K Morinaka; S Koyama; S Nakashima; T Hinoi; K Okawa; A Iwamatsu; A Kikuchi
Journal:  Oncogene       Date:  1999-10-21       Impact factor: 9.867

3.  Accelerated metabolism and exclusion of 4-hydroxynonenal through induction of RLIP76 and hGST5.8 is an early adaptive response of cells to heat and oxidative stress.

Authors:  J Z Cheng; R Sharma; Y Yang; S S Singhal; A Sharma; M K Saini; S V Singh; P Zimniak; S Awasthi; Y C Awasthi
Journal:  J Biol Chem       Date:  2001-08-24       Impact factor: 5.157

4.  Transfection of mGSTA4 in HL-60 cells protects against 4-hydroxynonenal-induced apoptosis by inhibiting JNK-mediated signaling.

Authors:  J Z Cheng; S S Singhal; A Sharma; M Saini; Y Yang; S Awasthi; P Zimniak; Y C Awasthi
Journal:  Arch Biochem Biophys       Date:  2001-08-15       Impact factor: 4.013

5.  Novel function of human RLIP76: ATP-dependent transport of glutathione conjugates and doxorubicin.

Authors:  S Awasthi; J Cheng; S S Singhal; M K Saini; U Pandya; S Pikula; J Bandorowicz-Pikula; S V Singh; P Zimniak; Y C Awasthi
Journal:  Biochemistry       Date:  2000-08-08       Impact factor: 3.162

Review 6.  Transport of glutathione conjugates and chemotherapeutic drugs by RLIP76 (RALBP1): a novel link between G-protein and tyrosine kinase signaling and drug resistance.

Authors:  Sanjay Awasthi; Sharad S Singhal; Rajendra Sharma; Piotr Zimniak; Yogesh C Awasthi
Journal:  Int J Cancer       Date:  2003-09-20       Impact factor: 7.396

7.  Regression of lung and colon cancer xenografts by depleting or inhibiting RLIP76 (Ral-binding protein 1).

Authors:  Sharad S Singhal; Jyotsana Singhal; Sushma Yadav; Seema Dwivedi; Paul J Boor; Yogesh C Awasthi; Sanjay Awasthi
Journal:  Cancer Res       Date:  2007-05-01       Impact factor: 12.701

8.  REPS2/POB1 is downregulated during human prostate cancer progression and inhibits growth factor signalling in prostate cancer cells.

Authors:  Josien K Oosterhoff; Fred Penninkhof; Albert O Brinkmann; J Anton Grootegoed; Leen J Blok
Journal:  Oncogene       Date:  2003-05-15       Impact factor: 9.867

9.  RLIP, an effector of the Ral GTPases, is a platform for Cdk1 to phosphorylate epsin during the switch off of endocytosis in mitosis.

Authors:  Carine Rossé; Sébastien L'Hoste; Nicolas Offner; André Picard; Jacques Camonis
Journal:  J Biol Chem       Date:  2003-05-29       Impact factor: 5.157

10.  Role of RLIP76 in lung cancer doxorubicin resistance: I. The ATPase activity of RLIP76 correlates with doxorubicin and 4-hydroxynonenal resistance in lung cancer cells.

Authors:  Sharad S Singhal; Jyotsana Singhal; Rajendra Sharma; Shivendra V Singh; Piotr Zimniak; Yogesh C Awasthi; Sanjay Awasthi
Journal:  Int J Oncol       Date:  2003-02       Impact factor: 5.650
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  15 in total

Review 1.  Antioxidant role of glutathione S-transferases: 4-Hydroxynonenal, a key molecule in stress-mediated signaling.

Authors:  Sharad S Singhal; Sharda P Singh; Preeti Singhal; David Horne; Jyotsana Singhal; Sanjay Awasthi
Journal:  Toxicol Appl Pharmacol       Date:  2015-10-23       Impact factor: 4.219

Review 2.  Role of RLIP76 in doxorubicin resistance in lung cancer.

Authors:  Rit Vatsyayan; Pankaj Chaudhary; Poorna Chandra Rao Lelsani; Preeti Singhal; Yogesh C Awasthi; Sanjay Awasthi; Sharad S Singhal
Journal:  Int J Oncol       Date:  2009-06       Impact factor: 5.650

Review 3.  Novel anti-cancer compounds for developing combinatorial therapies to target anoikis-resistant tumors.

Authors:  Lokesh Nagaprashantha; Neha Vartak; Sangeeta Awasthi; Sanjay Awasthi; Sharad S Singhal
Journal:  Pharm Res       Date:  2011-12-28       Impact factor: 4.200

4.  Knockdown of RLIP76 expression by RNA interference inhibits invasion, induces cell cycle arrest, and increases chemosensitivity to the anticancer drug temozolomide in glioma cells.

Authors:  Qi Wang; Jun Qian; Junyu Wang; Chun Luo; Juxiang Chen; Guohan Hu; Yicheng Lu
Journal:  J Neurooncol       Date:  2013-01-06       Impact factor: 4.130

Review 5.  RLIP76 Targeted Therapy for Kidney Cancer.

Authors:  Sharad S Singhal; Jyotsana Singhal; James Figarola; David Horne; Sanjay Awasthi
Journal:  Pharm Res       Date:  2015-05-29       Impact factor: 4.200

6.  Alpinumisoflavone suppresses tumour growth and metastasis of clear-cell renal cell carcinoma.

Authors:  Tingting Wang; Yuhua Jiang; Lei Chu; Tianhui Wu; Jie You
Journal:  Am J Cancer Res       Date:  2017-04-01       Impact factor: 6.166

7.  Glutathionylated 4-hydroxy-2-(E)-alkenal enantiomers in rat organs and their contributions toward the disposal of 4-hydroxy-2-(E)-nonenal in rat liver.

Authors:  Sushabhan Sadhukhan; Yong Han; Zhicheng Jin; Gregory P Tochtrop; Guo-Fang Zhang
Journal:  Free Radic Biol Med       Date:  2014-02-18       Impact factor: 7.376

8.  RALBP1/RLIP76 depletion in mice suppresses tumor growth by inhibiting tumor neovascularization.

Authors:  Seunghyung Lee; Jeremy G T Wurtzel; Sharad S Singhal; Sanjay Awasthi; Lawrence E Goldfinger
Journal:  Cancer Res       Date:  2012-08-17       Impact factor: 12.701

Review 9.  RLIP76: a versatile transporter and an emerging target for cancer therapy.

Authors:  Rit Vatsyayan; Poorna Chandra Rao Lelsani; Sanjay Awasthi; Sharad S Singhal
Journal:  Biochem Pharmacol       Date:  2010-01-25       Impact factor: 5.858

10.  A central role of RLIP76 in regulation of glycemic control.

Authors:  Sanjay Awasthi; Sharad S Singhal; Sushma Yadav; Jyotsana Singhal; Rit Vatsyayan; Ewa Zajac; Rafal Luchowski; Jozef Borvak; Karol Gryczynski; Yogesh C Awasthi
Journal:  Diabetes       Date:  2009-12-10       Impact factor: 9.461
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