PURPOSE: To assess the use of cellulose acetate filter rods as a technique for tear collection. METHOD: The cellulose acetate rod (CR) was compared with the 'standard' glass capillary tube (CT), in a series of experiments, to assess: sample collection by collected volume size; the effect of tear stimulation on total tear protein concentration and major tear protein concentrations; and technique invasiveness. RESULTS: No difference was found in concentrations for total protein, IgA (secretory immunoglobulin A), lactoferrin and lysozyme (p > 0.05) with no clinically significant increase in serum albumin to show serum leakage. Sample volume was higher for CR (p < 0.005) and sample volume increased for stimulated collection with CR (p = 0.001). Dilution effect of a stimulated sample size was reliably shown only with CR (r = -0.66, p = 0.011). Using bovine albumin standard with CR and CT, a smaller sample volume (p < 0.001) and a higher protein concentration (p < 0.001) were extracted with CR. CONCLUSION: The cellulose rod offers a suitable alternative to the glass CT. It is able to quickly absorb a sample, allowing use for a wide range of sample sizes, while being minimally invasive.
PURPOSE: To assess the use of cellulose acetate filter rods as a technique for tear collection. METHOD: The cellulose acetate rod (CR) was compared with the 'standard' glass capillary tube (CT), in a series of experiments, to assess: sample collection by collected volume size; the effect of tear stimulation on total tear protein concentration and major tear protein concentrations; and technique invasiveness. RESULTS: No difference was found in concentrations for total protein, IgA (secretory immunoglobulin A), lactoferrin and lysozyme (p > 0.05) with no clinically significant increase in serum albumin to show serum leakage. Sample volume was higher for CR (p < 0.005) and sample volume increased for stimulated collection with CR (p = 0.001). Dilution effect of a stimulated sample size was reliably shown only with CR (r = -0.66, p = 0.011). Using bovine albumin standard with CR and CT, a smaller sample volume (p < 0.001) and a higher protein concentration (p < 0.001) were extracted with CR. CONCLUSION: The cellulose rod offers a suitable alternative to the glass CT. It is able to quickly absorb a sample, allowing use for a wide range of sample sizes, while being minimally invasive.
Authors: Corey T Ungaro; Adam J Reimel; Ryan P Nuccio; Kelly A Barnes; Matthew D Pahnke; Lindsay B Baker Journal: Eur J Appl Physiol Date: 2015-01-10 Impact factor: 3.078
Authors: Aleksandra Inic-Kanada; Andrea Nussbaumer; Jacqueline Montanaro; Sandra Belij; Simone Schlacher; Elisabeth Stein; Nora Bintner; Margarethe Merio; Gerhard J Zlabinger; Talin Barisani-Asenbauer Journal: Mol Vis Date: 2012-11-16 Impact factor: 2.367