Literature DB >> 1907337

A specific fluorogenic assay for N-acetylgalactosamine-4-sulphatase activity using immunoadsorption.

D A Brooks1, G J Gibson, P A McCourt, J J Hopwood.   

Abstract

A method combining immune capture and enzyme detection by fluorochemistry has been developed for the diagnostic assay of N-acetylgalactosamine-4-sulphatase (4-sulphatase). The procedure uses a monoclonal antibody 4-S 4.1 to immunoadsorb 4-sulphatase specifically from complex protein samples containing other sulphatases, and 4-methylumbelliferyl sulphate to detect captured 4-sulphatase. The assay provides an accurate and simple method for the diagnosis of Maroteaux-Lamy syndrome (Mucopolysaccharidosis type VI).

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Year:  1991        PMID： 1907337     DOI： 10.1007/bf01804381

Source DB:  PubMed          Journal:  J Inherit Metab Dis        ISSN： 0141-8955            Impact factor:   4.982


  9 in total

Review 1.  Progress toward human gene therapy.

Authors:  T Friedmann
Journal:  Science       Date:  1989-06-16       Impact factor: 47.728

2.  Immunoquantification of the low abundance lysosomal enzyme N-acetylgalactosamine 4-sulphatase.

Authors:  D A Brooks; P A McCourt; G J Gibson; J J Hopwood
Journal:  J Inherit Metab Dis       Date:  1990       Impact factor: 4.982

3.  Diagnosis of Maroteaux-Lamy syndrome by the use of radiolabelled oligosaccharides as substrates for the determination of arylsulphatase B activity.

Authors:  J J Hopwood; H Elliott; V J Muller; G T Saccone
Journal:  Biochem J       Date:  1986-03-15       Impact factor: 3.857

4.  Differential assay of arylsulfatase A and B activities: a sensitive method for cultured human cells.

Authors:  P L Chang; N E Rosa; R G Davidson
Journal:  Anal Biochem       Date:  1981-11-01       Impact factor: 3.365

5.  Arylsulphatases A and B in human diploid fibroblasts: differential assay with 4-methylumbelliferylsulphate and AgNO3.

Authors:  R Mercelis; A F Van Elsen; J G Leroy
Journal:  Clin Chim Acta       Date:  1979-04-02       Impact factor: 3.786

6.  Synthesis and stability of arylsulfatase A and B in fibroblasts from multiple sulfatase deficiency.

Authors:  F Steckel; A Hasilik; K von Figura
Journal:  Eur J Biochem       Date:  1985-08-15

7.  Comparative activity of arylsulphatases A and B on two synthetic substrates.

Authors:  E E Delvin; A Pottier; F Glorieux
Journal:  Biochem J       Date:  1976-08-01       Impact factor: 3.857

8.  Inhibition of rabbit liver arylsulfatase B by phosphate esters.

Authors:  G J Rao; M E Christe
Journal:  Biochim Biophys Acta       Date:  1984-07-17

9.  Human N-acetylgalactosamine-4-sulphate sulphatase. Purification, monoclonal antibody production and native and subunit Mr values.

Authors:  G J Gibson; G T Saccone; D A Brooks; P R Clements; J J Hopwood
Journal:  Biochem J       Date:  1987-12-15       Impact factor: 3.857
  9 in total
  4 in total

Review 1.  Review: the immunochemical analysis of enzyme from mucopolysaccharidoses patients.

Authors:  D A Brooks
Journal:  J Inherit Metab Dis       Date:  1993       Impact factor: 4.982

2.  Correction of human mucopolysaccharidosis type-VI fibroblasts with recombinant N-acetylgalactosamine-4-sulphatase.

Authors:  D S Anson; J A Taylor; J Bielicki; G S Harper; C Peters; G J Gibson; J J Hopwood
Journal:  Biochem J       Date:  1992-06-15       Impact factor: 3.857

3.  Overexpression of N-acetylgalactosamine-4-sulphatase induces a multiple sulphatase deficiency in mucopolysaccharidosis-type-VI fibroblasts.

Authors:  D S Anson; V Muller; J Bielicki; G S Harper; J J Hopwood
Journal:  Biochem J       Date:  1993-09-15       Impact factor: 3.857

4.  Two site-directed mutations abrogate enzyme activity but have different effects on the conformation and cellular content of the N-acetylgalactosamine 4-sulphatase protein.

Authors:  D A Brooks; D A Robertson; C Bindloss; T Litjens; D S Anson; C Peters; C P Morris; J J Hopwood
Journal:  Biochem J       Date:  1995-04-15       Impact factor: 3.857
  4 in total

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