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Literature DB >> 19066811

Enumeration of bacteriophages by double agar overlay plaque assay.

Andrew M Kropinski¹, Amanda Mazzocco, Thomas E Waddell, Erika Lingohr, Roger P Johnson.

Abstract

The determination of the concentration of infectious phage particles is fundamental to many protocols in phage biology, genetics, and molecular biology. In this chapter the classical overlay protocol is described.

Entities: Chemical

Mesh：

Substances：
Agar

Year: 2009 PMID： 19066811 DOI： 10.1007/978-1-60327-164-6_7

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

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Cited

190 in total

Review 1. Listeria phages: Genomes, evolution, and application.

Authors: Jochen Klumpp; Martin J Loessner
Journal: Bacteriophage Date: 2013-10-24

2. Phage infection of an environmentally relevant marine bacterium alters host metabolism and lysate composition.

Authors: Nana Yaw D Ankrah; Amanda L May; Jesse L Middleton; Daniel R Jones; Mary K Hadden; Jessica R Gooding; Gary R LeCleir; Steven W Wilhelm; Shawn R Campagna; Alison Buchan
Journal: ISME J Date: 2013-12-05 Impact factor: 10.302

3. Quantification of diverse virus populations in the environment using the polony method.

Authors: Nava Baran; Svetlana Goldin; Ilia Maidanik; Debbie Lindell
Journal: Nat Microbiol Date: 2017-10-30 Impact factor: 17.745

4. UVC LED Irradiation Effectively Inactivates Aerosolized Viruses, Bacteria, and Fungi in a Chamber-Type Air Disinfection System.

Authors: Do-Kyun Kim; Dong-Hyun Kang
Journal: Appl Environ Microbiol Date: 2018-08-17 Impact factor: 4.792

Enumeration of bacteriophages by double agar overlay plaque assay.

Review 1. Listeria phages: Genomes, evolution, and application.

2. Phage infection of an environmentally relevant marine bacterium alters host metabolism and lysate composition.

3. Quantification of diverse virus populations in the environment using the polony method.

4. UVC LED Irradiation Effectively Inactivates Aerosolized Viruses, Bacteria, and Fungi in a Chamber-Type Air Disinfection System.

5. Phage-Antibiotic Synergy via Delayed Lysis.

6. Genome analysis of Salmonella phage vB_SalM_8-19 (genus Rosemountvirus).

7. The Effects of Statistical Multiplicity of Infection on Virus Quantification and Infectivity Assays.

8. Mutational Analysis of the Antitoxin in the Lactococcal Type III Toxin-Antitoxin System AbiQ.

9. Bacteriophage application for biocontrolling Shigella flexneri in contaminated foods.

10. The use of antibiotics to improve phage detection and enumeration by the double-layer agar technique.