Literature DB >> 19066550

Counting and determining the viability of cultured cells.

Richard Ricardo1, Katy Phelan.   

Abstract

Determining the number of cells in culture is important in standardization of culture conditions and in performing accurate quantitation experiments. A hemacytometer is a thick glass slide with a central area designed as a counting chamber. Cell suspension is applied to a defined area and counted so cell density can be calculated.

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Year:  2008        PMID: 19066550      PMCID: PMC2583037          DOI: 10.3791/752

Source DB:  PubMed          Journal:  J Vis Exp        ISSN: 1940-087X            Impact factor:   1.355


  8 in total

1.  Isolation and large scale expansion of adult human endothelial colony forming progenitor cells.

Authors:  Nicole A Hofmann; Andreas Reinisch; Dirk Strunk
Journal:  J Vis Exp       Date:  2009-10-28       Impact factor: 1.355

2.  Automated Quantification and Analysis of Cell Counting Procedures Using ImageJ Plugins.

Authors:  Jacob O'Brien; Heyam Hayder; Chun Peng
Journal:  J Vis Exp       Date:  2016-11-17       Impact factor: 1.355

3.  Microcrystallography of Protein Crystals and In Cellulo Diffraction.

Authors:  Marion Boudes; Damià Garriga; Fasséli Coulibaly
Journal:  J Vis Exp       Date:  2017-07-21       Impact factor: 1.355

4.  Counting unstained, confluent cells by modified bright-field microscopy.

Authors:  L Louis Drey; Michael C Graber; Jan Bieschke
Journal:  Biotechniques       Date:  2013-07       Impact factor: 1.993

5.  An automated cell-counting algorithm for fluorescently-stained cells in migration assays.

Authors:  Baraa K Al-Khazraji; Philip J Medeiros; Nicole M Novielli; Dwayne N Jackson
Journal:  Biol Proced Online       Date:  2011-10-19       Impact factor: 3.244

6.  Escherichia coli STb enterotoxin dislodges claudin-1 from epithelial tight junctions.

Authors:  Hassan Nassour; J Daniel Dubreuil
Journal:  PLoS One       Date:  2014-11-19       Impact factor: 3.240

7.  Optical microscopic study of surface morphology and filtering efficiency of face masks.

Authors:  Bhanu Bhakta Neupane; Sangita Mainali; Amita Sharma; Basant Giri
Journal:  PeerJ       Date:  2019-06-26       Impact factor: 2.984

8.  M2 Muscarinic acetylcholine receptor modulates rat airway smooth muscle cell proliferation.

Authors:  Fabiola A Placeres-Uray; Christopher A Febres-Aldana; Ruth Fernandez-Ruiz; Ramona Gonzalez de Alfonzo; Itala A Lippo de Becemberg; Marcelo J Alfonzo
Journal:  World Allergy Organ J       Date:  2013-12-30       Impact factor: 4.084

  8 in total

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