| Literature DB >> 19062065 |
Zdena Kohoutová1, Michaela Rumlová, Martin Andreánsky, Michael Sakalian, Eric Hunter, Iva Pichová, Tomás Ruml.
Abstract
Most retroviruses employ a frameshift mechanism during polyprotein synthesis to balance appropriate ratios of structural proteins and enzymes. To investigate the requirements for individual precursors in retrovirus assembly, we modified the polyprotein repertoire of Mason-Pfizer monkey virus (M-PMV) by mutating the frameshift sites to imitate the polyprotein organization of Rous sarcoma virus (Gag-Pro and Gag-Pro-Pol) or Human immunodeficiency virus (Gag and Gag-Pro-Pol). For the "Rous-like" virus, assembly was impaired with no incorporation of Gag-Pro-Pol into particles and for the "HIV-like" virus an altered morphogenesis was observed. A mutant expressing Gag and Gag-Pro polyproteins and lacking Gag-Pro-Pol assembled intracellular particles at a level similar to the wild-type. Gag-Pro-Pol polyprotein alone neither formed immature particles nor processed the precursor. All the mutants were non-infectious except the "HIV-like", which retained fractional infectivity.Entities:
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Year: 2008 PMID： 19062065 PMCID： PMC3779691 DOI： 10.1016/j.virol.2008.10.048
Source DB: PubMed Journal: Virology ISSN： 0042-6822 Impact factor: 3.616