| Literature DB >> 19059484 |
M Dewilde1, S V Strelkov, A Rabijns, P J Declerck.
Abstract
Here we report the crystal structure of a stablilized plasminogen activator inhibitor-1 variant (PAI-1-N150H-K154T-Q301P-Q319L-M354I (PAI-1-stab)) that shows a cleavage within the reactive centre loop. The new structure is of superior quality compared to the previously determined structure of the cleaved PAI-1-A335P mutant. We present a detailed comparison of the two structures and also compare them with the structure of the active PAI-1-stab. The structural data give important insights into the working mechanism of PAI-1 and also explain the role of various stabilizing mutations.Entities:
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Year: 2008 PMID： 19059484 DOI： 10.1016/j.jsb.2008.11.001
Source DB: PubMed Journal: J Struct Biol ISSN： 1047-8477 Impact factor: 2.867