Dong-Feng Chen1, Lu Hu, Ping Yi, Wei-Wen Liu, Dian-Chun Fang, Hong Cao. 1. Department of Gastroenterology, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China. dfchen9@hotmail.com.cn
Abstract
AIM: To study the mechanism by which Helicobacter pylori (H pylori) damages human gallbladder epithelial cells (HGBEC). METHODS: H pylori isolated from gallbladder were cultured in a liquid medium. Different concentration supernatants and sonicated extracts of H pylori cells were then added to HGBEC in a primary culture. The morphological changes in HGBEC as well as changes in the levels of alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and glutamyltransferase (GGT) were measured. RESULTS: According to the culture curve of HGBEC, it was convenient to study the changes in HGBEC by adding H pylori sonicated extracts and H pylori culture supernatants. Both H pylori sonicated extracts and H pylori culture supernatants had a significant influence on HGBEC morphology, i.e. HGBEC grew more slowly, their viability decreased and their detachment increased. Furthermore, HGBEC ruptured and died. The levels of ALP (33.84+/-6.00 vs 27.01+/-4.67, P<0.05), LDH (168.37+/-20.84 vs 55.51+/-17.17, P<0.01) and GGT (42.01+/-6.18 vs 25.34+/-4.33, P<0.01) significantly increased in the HGBEC culture supernatant in a time- and concentration-dependent. The damage to HGBEC in H pylori culture liquid was more significant than that in H pylori sonicated extracts. CONCLUSION: H pylori induces no obvious damage to HGBEC.
AIM: To study the mechanism by which Helicobacter pylori (H pylori) damages human gallbladder epithelial cells (HGBEC). METHODS:H pylori isolated from gallbladder were cultured in a liquid medium. Different concentration supernatants and sonicated extracts of H pylori cells were then added to HGBEC in a primary culture. The morphological changes in HGBEC as well as changes in the levels of alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and glutamyltransferase (GGT) were measured. RESULTS: According to the culture curve of HGBEC, it was convenient to study the changes in HGBEC by adding H pylori sonicated extracts and H pylori culture supernatants. Both H pylori sonicated extracts and H pylori culture supernatants had a significant influence on HGBEC morphology, i.e. HGBEC grew more slowly, their viability decreased and their detachment increased. Furthermore, HGBEC ruptured and died. The levels of ALP (33.84+/-6.00 vs 27.01+/-4.67, P<0.05), LDH (168.37+/-20.84 vs 55.51+/-17.17, P<0.01) and GGT (42.01+/-6.18 vs 25.34+/-4.33, P<0.01) significantly increased in the HGBEC culture supernatant in a time- and concentration-dependent. The damage to HGBEC in H pylori culture liquid was more significant than that in H pylori sonicated extracts. CONCLUSION:H pylori induces no obvious damage to HGBEC.
Authors: Reinhard K Straubinger; Andrea Greiter; Sean P McDonough; Alexander Gerold; Eugenio Scanziani; Sabina Soldati; Daiva Dailidiene; Giedrius Dailide; Douglas E Berg; Kenneth W Simpson Journal: Infect Immun Date: 2003-05 Impact factor: 3.441
Authors: Bruno Neu; Pamela Randlkofer; Mathilde Neuhofer; Petra Voland; Artur Mayerhofer; Markus Gerhard; Wolfgang Schepp; Christian Prinz Journal: Am J Physiol Gastrointest Liver Physiol Date: 2002-08 Impact factor: 4.052