AIM: To analyze the association between Helicobacter spp. and some common gut bacteria in patients with cholecystitis. METHODS: A nested-polymerase chain reaction (PCR), specific to 16S rRNA of Helicobacter spp. was performed on paraffin-embedded gallbladder samples of 100 cholecystitis and 102 control cases. The samples were also analyzed for some common gut bacteria by PCR. Positive samples were sequenced for species identification. RESULTS: Helicobacter DNA was found in seven out of 100 cases of acute and chronic cholecystitis. Sequence analysis displayed Helicobacter pullorum (H. pullorum) in six cases and Helicobacter pylori in one; H. pullorum was only found in cases with metaplasia. Control samples were negative for Helicobacter spp. and some common gut bacteria. There was a significant difference (P = 0.007) between cholecystitis and control samples for Helicobacter DNA. CONCLUSION: A possible relationship was detected between Helicobacter DNA and cholecystitis. Further serological and immunohistochemical studies are needed to support these data.
AIM: To analyze the association between Helicobacter spp. and some common gut bacteria in patients with cholecystitis. METHODS: A nested-polymerase chain reaction (PCR), specific to 16S rRNA of Helicobacter spp. was performed on paraffin-embedded gallbladder samples of 100 cholecystitis and 102 control cases. The samples were also analyzed for some common gut bacteria by PCR. Positive samples were sequenced for species identification. RESULTS:Helicobacter DNA was found in seven out of 100 cases of acute and chronic cholecystitis. Sequence analysis displayed Helicobacter pullorum (H. pullorum) in six cases and Helicobacter pylori in one; H. pullorum was only found in cases with metaplasia. Control samples were negative for Helicobacter spp. and some common gut bacteria. There was a significant difference (P = 0.007) between cholecystitis and control samples for Helicobacter DNA. CONCLUSION: A possible relationship was detected between Helicobacter DNA and cholecystitis. Further serological and immunohistochemical studies are needed to support these data.
Authors: Milutin Bulajic; Patrick Maisonneuve; Wulf Schneider-Brachert; Petra Müller; Udo Reischl; Bojan Stimec; Norbert Lehn; Albert B Lowenfels; Matthias Löhr Journal: Cancer Date: 2002-11-01 Impact factor: 6.860
Authors: S J Myung; M H Kim; K N Shim; Y S Kim; E O Kim; H J Kim; E T Park; K S Yoo; B C Lim; D W Seo; S K Lee; Y I Min; J Y Kim Journal: Dig Dis Sci Date: 2000-07 Impact factor: 3.199
Authors: Kirk J Maurer; Arlin B Rogers; Zhongming Ge; Ashley J Wiese; Martin C Carey; James G Fox Journal: Am J Physiol Gastrointest Liver Physiol Date: 2005-08-18 Impact factor: 4.052