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Literature DB >> 1905660

Construction of scrA::lacZ gene fusions to investigate regulation of the sucrose PTS of Streptococcus mutans.

Y Sato¹, Y Yamamoto, R Suzuki, H Kizaki, H K Kuramitsu.

Abstract

The scrA gene coding for sucrose EnzymeII of the phosphoenolpyruvate dependent phosphotransferase system previously isolated from Streptococcus mutans was fused in vitro to the promoterless lacZ' gene to monitor the expression of the scrA gene. The scrA::lacZ gene fusion was introduced back into S. mutans GS-5IS3 by two independent transformation procedures involving either linear or plasmid DNA to produce both scrA and scrA+ mutants. These mutants should prove useful for analyzing the regulation of sucrose transport in S. mutans.

Entities: Chemical Species

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Year: 1991 PMID： 1905660 DOI： 10.1111/j.1574-6968.1991.tb04552.x

Source DB: PubMed Journal: FEMS Microbiol Lett ISSN： 0378-1097 Impact factor: 2.742

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Cited

9 in total

1. Roles of fructosyltransferase and levanase-sucrase of Actinomyces naeslundii in fructan and sucrose metabolism.

Authors: L J Bergeron; R A Burne
Journal: Infect Immun Date: 2001-09 Impact factor: 3.441

2. Cloning and sequence analysis of the gbpC gene encoding a novel glucan-binding protein of Streptococcus mutans.

Authors: Y Sato; Y Yamamoto; H Kizaki
Journal: Infect Immun Date: 1997-02 Impact factor: 3.441

3. Development of a system for expressing heterologous genes in the oral spirochete Treponema denticola and its use in expression of the Treponema pallidum flaA gene.

Authors: B Chi; S Chauhan; H Kuramitsu
Journal: Infect Immun Date: 1999-07 Impact factor: 3.441

4. Disruption of the adh (Acetoin Dehydrogenase) Operon Has Wide-Ranging Effects on Streptococcus mutans Growth and Stress Response.

Authors: Peter Zuber; Michiko M Nakano; Jessica K Kajfasz; José A Lemos
Journal: J Bacteriol Date: 2022-01-10 Impact factor: 3.476

5. Control of enzyme IIscr and sucrose-6-phosphate hydrolase activities in Streptococcus mutans by transcriptional repressor ScrR binding to the cis-active determinants of the scr regulon.

Authors: Bing Wang; Howard K Kuramitsu
Journal: J Bacteriol Date: 2003-10 Impact factor: 3.490

Construction of scrA::lacZ gene fusions to investigate regulation of the sucrose PTS of Streptococcus mutans.

1. Roles of fructosyltransferase and levanase-sucrase of Actinomyces naeslundii in fructan and sucrose metabolism.

2. Cloning and sequence analysis of the gbpC gene encoding a novel glucan-binding protein of Streptococcus mutans.

3. Development of a system for expressing heterologous genes in the oral spirochete Treponema denticola and its use in expression of the Treponema pallidum flaA gene.

4. Disruption of the adh (Acetoin Dehydrogenase) Operon Has Wide-Ranging Effects on Streptococcus mutans Growth and Stress Response.

5. Control of enzyme IIscr and sucrose-6-phosphate hydrolase activities in Streptococcus mutans by transcriptional repressor ScrR binding to the cis-active determinants of the scr regulon.

6. Regulation of sucrose-6-phosphate hydrolase activity in Streptococcus mutans: characterization of the scrR gene.

7. Characterization of a novel methyl-accepting chemotaxis gene, dmcB, from the oral spirochete Treponema denticola.

8. A mechanism for extremely weak SpaP-expression in Streptococcus mutans strain Z1.

9. The malQ gene is essential for starch metabolism in Streptococcus mutans.