Literature DB >> 10377154

Development of a system for expressing heterologous genes in the oral spirochete Treponema denticola and its use in expression of the Treponema pallidum flaA gene.

B Chi1, S Chauhan, H Kuramitsu.   

Abstract

The present communication describes the construction of a new Escherichia coli-Treponema denticola shuttle vector based on the naturally occurring spirochete plasmid pTS1 and the expression of the heterologous T. pallidum flaA gene from the plasmid in T. denticola. This new shuttle vector system should prove useful in characterizing virulence factors from unculturable pathogenic spirochetes.

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Year:  1999        PMID： 10377154      PMCID： PMC116559     

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  14 in total

1.  Characterization of a 4.2-kb plasmid isolated from periodontopathic spirochetes.

Authors:  E C Chan; A Klitorinos; S Gharbia; S D Caudry; M D Rahal; R Siboo
Journal:  Oral Microbiol Immunol       Date:  1996-10

2.  Expression in Escherichia coli of the 37-kilodalton endoflagellar sheath protein of Treponema pallidum by use of the polymerase chain reaction and a T7 expression system.

Authors:  R D Isaacs; J D Radolf
Journal:  Infect Immun       Date:  1990-07       Impact factor: 3.441

3.  Relative distribution of bacteria at clinically healthy and periodontally diseased sites in humans.

Authors:  M A Listgarten; L Helldén
Journal:  J Clin Periodontol       Date:  1978-05       Impact factor: 8.728

4.  Quantitative relationship of Treponema denticola to severity of periodontal disease.

Authors:  L G Simonson; C H Goodman; J J Bial; H E Morton
Journal:  Infect Immun       Date:  1988-04       Impact factor: 3.441

5.  Isolation and characterization of a plasmid from Treponema denticola.

Authors:  A Ivic; J MacDougall; R R Russell; C W Penn
Journal:  FEMS Microbiol Lett       Date:  1991-03-01       Impact factor: 2.742

6.  Cloning and sequence analysis of a chymotrypsinlike protease from Treponema denticola.

Authors:  S Arakawa; H K Kuramitsu
Journal:  Infect Immun       Date:  1994-08       Impact factor: 3.441

7.  Complete nucleotide sequence of a Selenomonas ruminantium plasmid and definition of a region necessary for its replication in Escherichia coli.

Authors:  G T Attwood; J D Brooker
Journal:  Plasmid       Date:  1992-09       Impact factor: 3.466

8.  Characterization of the 35-kilodalton Treponema pallidum subsp. pallidum recombinant lipoprotein TmpC and antibody response to lipidated and nonlipidated T. pallidum antigens.

Authors:  L M Schouls; H G van der Heide; J D van Embden
Journal:  Infect Immun       Date:  1991-10       Impact factor: 3.441

9.  Characterization of monoclonal antibodies to Treponema pallidum.

Authors:  S A Lukehart; M R Tam; J Hom; S A Baker-Zander; K K Holmes; R C Nowinski
Journal:  J Immunol       Date:  1985-01       Impact factor: 5.422

10.  Molecular cloning and DNA sequence analysis of the 37-kilodalton endoflagellar sheath protein gene of Treponema pallidum.

Authors:  R D Isaacs; J H Hanke; L M Guzman-Verduzco; G Newport; N Agabian; M V Norgard; S A Lukehart; J D Radolf
Journal:  Infect Immun       Date:  1989-11       Impact factor: 3.441
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  21 in total

Review 1.  Spirochaetal lipoproteins and pathogenesis.

Authors:  D A Haake
Journal:  Microbiology       Date:  2000-07       Impact factor: 2.777

Review 2.  Biological basis for syphilis.

Authors:  Rebecca E Lafond; Sheila A Lukehart
Journal:  Clin Microbiol Rev       Date:  2006-01       Impact factor: 26.132

3.  Construction and characterization of a cheA mutant of Treponema denticola.

Authors:  Renate Lux; Jee-Hyun Sim; Jon P Tsai; Wenyuan Shi
Journal:  J Bacteriol       Date:  2002-06       Impact factor: 3.490

4.  Culture-enriched metagenomic sequencing enables in-depth profiling of the cystic fibrosis lung microbiota.

Authors:  Fiona J Whelan; Barbara Waddell; Saad A Syed; Shahrokh Shekarriz; Harvey R Rabin; Michael D Parkins; Michael G Surette
Journal:  Nat Microbiol       Date:  2020-01-20       Impact factor: 17.745

5.  Disruption of a type II endonuclease (TDE0911) enables Treponema denticola ATCC 35405 to accept an unmethylated shuttle vector.

Authors:  Jiang Bian; Chunhao Li
Journal:  Appl Environ Microbiol       Date:  2011-05-20       Impact factor: 4.792

6.  The LE1 bacteriophage replicates as a plasmid within Leptospira biflexa: construction of an L. biflexa-Escherichia coli shuttle vector.

Authors:  I S Girons; P Bourhy; C Ottone; M Picardeau; D Yelton; R W Hendrix; P Glaser; N Charon
Journal:  J Bacteriol       Date:  2000-10       Impact factor: 3.490

7.  A novel glycan modifies the flagellar filament proteins of the oral bacterium Treponema denticola.

Authors:  Kurni Kurniyati; John F Kelly; Evgeny Vinogradov; Anna Robotham; Youbing Tu; Juyu Wang; Jun Liu; Susan M Logan; Chunhao Li
Journal:  Mol Microbiol       Date:  2016-10-27       Impact factor: 3.501

8.  Development of a novel chloramphenicol resistance expression plasmid used for genetic complementation of a fliG deletion mutant in Treponema denticola.

Authors:  Linda L Slivienski-Gebhardt; Jacques Izard; William A Samsonoff; Ronald J Limberger
Journal:  Infect Immun       Date:  2004-09       Impact factor: 3.441

9.  Multiple functions of the leucine-rich repeat protein LrrA of Treponema denticola.

Authors:  Akihiko Ikegami; Kiyonobu Honma; Ashu Sharma; Howard K Kuramitsu
Journal:  Infect Immun       Date:  2004-08       Impact factor: 3.441

10.  Genetic Manipulations of Oral Spirochete Treponema denticola.

Authors:  Kurni Kurniyati; Chunhao Li
Journal:  Methods Mol Biol       Date:  2021
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