| Literature DB >> 19053386 |
Maher Chaouachi1, Gaëlle Chupeau, Aurélie Berard, Heather McKhann, Marcel Romaniuk, Sandra Giancola, Valérie Laval, Yves Bertheau, Dominique Brunel.
Abstract
A high-throughput multiplex assay for the detection of genetically modified organisms (GMO) was developed on the basis of the existing SNPlex method designed for SNP genotyping. This SNPlex assay allows the simultaneous detection of up to 48 short DNA sequences (approximately 70 bp; "signature sequences") from taxa endogenous reference genes, from GMO constructions, screening targets, construct-specific, and event-specific targets, and finally from donor organisms. This assay avoids certain shortcomings of multiplex PCR-based methods already in widespread use for GMO detection. The assay demonstrated high specificity and sensitivity. The results suggest that this assay is reliable, flexible, and cost- and time-effective for high-throughput GMO detection.Entities:
Mesh：
Year: 2008 PMID： 19053386 DOI： 10.1021/jf801482r
Source DB: PubMed Journal: J Agric Food Chem ISSN： 0021-8561 Impact factor: 5.279