PURPOSE: To investigate the short term effects of Doxycycline on MMP-2 and MMP-9. METHODS: Short term effects of Doxycycline (100 mg B.I.D.) on plasma levels of MMP-2 and MMP-9 were investigated in 20 healthy subjects; the effects of Doxy, Acetylsalicylic acid, Nitrates, and Enalapril on MMP-9 release from were assessed in isolated polymorphonuclear cells. RESULTS: In plasma, MMP-9 activity was reduced (-22%, 95% CI -32/-11; P = 0.002) starting at 12 h after doxy; in vitro, MMP-9 released from stimulated neutrophils was reduced by Doxy (-28%, 95% CI -43/-14; P = 0.001), inhibiting degranulation, and by nitrates (-52%, 95% CI -76/-28 P = 0.005), increasing three times both pro- and active-MMP-9 bound to neutrophils (P = 0.007 and 0.040, respectively). CONCLUSIONS: Doxy decreases MMP-9 plasma levels by around 20%, within the first 12 h. The mechanism leading to such reduction seems due to the inhibition of PMN degranulation.
PURPOSE: To investigate the short term effects of Doxycycline on MMP-2 and MMP-9. METHODS: Short term effects of Doxycycline (100 mg B.I.D.) on plasma levels of MMP-2 and MMP-9 were investigated in 20 healthy subjects; the effects of Doxy, Acetylsalicylic acid, Nitrates, and Enalapril on MMP-9 release from were assessed in isolated polymorphonuclear cells. RESULTS: In plasma, MMP-9 activity was reduced (-22%, 95% CI -32/-11; P = 0.002) starting at 12 h after doxy; in vitro, MMP-9 released from stimulated neutrophils was reduced by Doxy (-28%, 95% CI -43/-14; P = 0.001), inhibiting degranulation, and by nitrates (-52%, 95% CI -76/-28 P = 0.005), increasing three times both pro- and active-MMP-9 bound to neutrophils (P = 0.007 and 0.040, respectively). CONCLUSIONS:Doxy decreases MMP-9 plasma levels by around 20%, within the first 12 h. The mechanism leading to such reduction seems due to the inhibition of PMN degranulation.
Authors: Kathryn M Thrailkill; Cynthia S Moreau; Gael E Cockrell; Chan-Hee Jo; Robert C Bunn; Alba E Morales-Pozzo; Charles K Lumpkin; John L Fowlkes Journal: Endocrine Date: 2010-01-12 Impact factor: 3.633
Authors: Prashant S Dalvi; Anil Singh; Hiren R Trivedi; Feroz D Ghanchi; Dinesh M Parmar; Suresh D Mistry Journal: Ann Thorac Med Date: 2011-10 Impact factor: 2.219
Authors: Philippe Eloy; Anne Lise Poirrier; Clotilde De Dorlodot; Thibaut Van Zele; Jean Baptiste Watelet; Bernard Bertrand Journal: Curr Allergy Asthma Rep Date: 2011-04 Impact factor: 4.806
Authors: Nicola Fiotti; Filippo Mearelli; Filippo Giorgio Di Girolamo; Luigi Mario Castello; Alessio Nunnari; Salvatore Di Somma; Enrico Lupia; Efrem Colonetti; Maria Lorenza Muiesan; Giuseppe Montrucchio; Carlo Giansante; Gian Carlo Avanzi; Gianni Biolo Journal: Biomolecules Date: 2022-02-09