| Literature DB >> 19052379 |
Tatsuro Shimamura1, Shunsuke Yajima, Shun'ichi Suzuki, Nicholas G Rutherford, John O'Reilly, Peter J F Henderson, So Iwata.
Abstract
The integral membrane protein Mhp1 from Microbacterium liquefaciens transports hydantoins and belongs to the nucleobase:cation symporter 1 family. Mhp1 was successfully purified and crystallized. Initial crystals were obtained using the hanging-drop vapour-diffusion method but diffracted poorly. Optimization of the crystallization conditions resulted in the generation of orthorhombic crystals (space group P2(1)2(1)2(1), unit-cell parameters a = 79.7, b = 101.1, c = 113.8 A). A complete data set has been collected from a single crystal to a resolution of 2.85 A with 64 741 independent observations (94% complete) and an R(merge) of 0.12. Further experimental phasing methods are under way.Entities:
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Year: 2008 PMID： 19052379 PMCID： PMC2593711 DOI： 10.1107/S1744309108036920
Source DB: PubMed Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun ISSN： 1744-3091