| Literature DB >> 19048215 |
Jorge Carneiro1, Lurdes Duarte, Elisabetta Padovan.
Abstract
Limiting dilution analysis (LDA) has been extensively employed as a quantitative method to estimate the precursor frequency of various T lymphocyte subsets according to their functional properties in vitro. We describe here an example of LDA experiment assessing antigen-specific T cell proliferation of microcultures in the presence or absence of adjuvant and illustrate how to estimate the frequencies of precursor T cells using an online tool that we made publicly available.Mesh：
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Year: 2009 PMID： 19048215 DOI： 10.1007/978-1-60327-527-9_7
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745