AIM OF THE STUDY: To determine the anti-inflammatory, analgesic and antioxidant activities of the leaves of Memecylon edule Roxb. used traditionally in Thailand. MATERIALS AND METHODS: Hexane, (Hex), ethyl acetate (EtOAc), methanol (MeOH) and 50% methanol (MeOH50) fractions of the dry leaves were tested in vitro for their interleukin-10 production; the most active fraction was further studied in vivo for its anti-inflammatory and analgesic activities using the ethylphenylpropiolate (EPP)-induced mouse ear edema and the writhing test with mice. All fractions except Hex were tested for their radical scavenging activity towards 1'-diphenyl-2-picrylhydrazyl radical (DPPH). RESULTS: The EtOAc showed the highest stimulation for interleukin-10 production. In the EPP test, this fraction was significantly active 30 min after topical application at all doses used (0.5, 1.0, 2.0mg/ear); after 4h and at 1.0mg/ear EtOAc was slightly less active (inhibition 47.8%) than the reference, indomethacin, at the same dose (62.4%). At 200mg/kg orally, the EtOAc caused a significant inhibition of the writhing response by 56.6% which was like indomethacin at 10mg/kg. EtOAc, MeOH and MeOH50 exhibited radical scavenging activity. The order of IC(50) values was: ascorbic acid (9.1 microg/mL)>trolox (11.6 microg/mL)>MeOH (46.9 microg/mL)>MeOH50 (152.1 microg/mL)>EtOAc (1742.2 microg/mL). CONCLUSION: The results provide support for the traditional use of Memecylon edule leaves in relieving inflammation and pain.
AIM OF THE STUDY: To determine the anti-inflammatory, analgesic and antioxidant activities of the leaves of Memecylon edule Roxb. used traditionally in Thailand. MATERIALS AND METHODS:Hexane, (Hex), ethyl acetate (EtOAc), methanol (MeOH) and 50% methanol (MeOH50) fractions of the dry leaves were tested in vitro for their interleukin-10 production; the most active fraction was further studied in vivo for its anti-inflammatory and analgesic activities using the ethylphenylpropiolate (EPP)-induced mouse ear edema and the writhing test with mice. All fractions except Hex were tested for their radical scavenging activity towards 1'-diphenyl-2-picrylhydrazyl radical (DPPH). RESULTS: The EtOAc showed the highest stimulation for interleukin-10 production. In the EPP test, this fraction was significantly active 30 min after topical application at all doses used (0.5, 1.0, 2.0mg/ear); after 4h and at 1.0mg/ear EtOAc was slightly less active (inhibition 47.8%) than the reference, indomethacin, at the same dose (62.4%). At 200mg/kg orally, the EtOAc caused a significant inhibition of the writhing response by 56.6% which was like indomethacin at 10mg/kg. EtOAc, MeOH and MeOH50 exhibited radical scavenging activity. The order of IC(50) values was: ascorbic acid (9.1 microg/mL)>trolox (11.6 microg/mL)>MeOH (46.9 microg/mL)>MeOH50 (152.1 microg/mL)>EtOAc (1742.2 microg/mL). CONCLUSION: The results provide support for the traditional use of Memecylon edule leaves in relieving inflammation and pain.
Authors: Aline Stamford S G Gatis-Carrazzoni; Fernanda Virgínia Barreto Mota; Tonny Cley Campos Leite; Tatiane Bezerra de Oliveira; Sandra Cabral da Silva; Isla Vanessa Alves Bastos; Maria Bernadete de Souza Maia; Pedro Silvino Pereira; Pedro Paulo Marcelino Neto; Earl Celestino de Oliveira Chagas; Tania Maria Sarmento Silva; Márcia Silva do Nascimento; Teresinha Gonçalves da Silva Journal: Naunyn Schmiedebergs Arch Pharmacol Date: 2018-09-13 Impact factor: 3.000
Authors: Permal Deo; Erandi Hewawasam; Aris Karakoulakis; David J Claudie; Robert Nelson; Bradley S Simpson; Nicholas M Smith; Susan J Semple Journal: BMC Complement Altern Med Date: 2016-11-04 Impact factor: 3.659