Literature DB >> 1904084

Macrophage NO2- production as a sensitive and rapid assay for the quantitation of murine IFN-gamma.

P Migliorini1, G Corradin, S B Corradin.   

Abstract

Macrophage production of arginine-derived NO2- provides a simple method for detection and quantitation of interferon-gamma (IFN-gamma) in murine cell culture fluids. When the macrophage cell line RAW 264 is cultured overnight with IFN-gamma in the presence of 10 ng/ml LPS, NO2- release, as determined by a simple colorimetric assay, is proportional to the concentration of IFN-gamma and is inhibited by monoclonal antibody to IFN-gamma. A high degree of correlation was obtained when antigen stimulated T lymphocyte supernatants were tested for IFN-gamma by ELISA and the NO2- production assay.

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Year:  1991        PMID： 1904084     DOI： 10.1016/0022-1759(91)90357-l

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  17 in total

1.  The gills are an important site of iNOS expression in rainbow trout Oncorhynchus mykiss after challenge with the gram-positive pathogen Renibacterium salmoninarum.

Authors:  J J Campos-Perez; M Ward; P S Grabowski; A E Ellis; C J Secombes
Journal:  Immunology       Date:  2000-01       Impact factor: 7.397

2.  Nitric oxide contributes to host resistance against experimental Taenia crassiceps cysticercosis.

Authors:  Javier Alonso-Trujillo; Irma Rivera-Montoya; Miriam Rodríguez-Sosa; Luis I Terrazas
Journal:  Parasitol Res       Date:  2007-01-06       Impact factor: 2.289

3.  Nitric oxide is produced by Cowdria ruminantium-infected bovine pulmonary endothelial cells in vitro and is stimulated by gamma interferon.

Authors:  M Mutunga; P M Preston; K J Sumption
Journal:  Infect Immun       Date:  1998-05       Impact factor: 3.441

4.  Effect of MALP-2, a lipopeptide from Mycoplasma fermentans, on bone resorption in vitro.

Authors:  G Piec; J Mirkovitch; S Palacio; P F Mühlradt; R Felix
Journal:  Infect Immun       Date:  1999-12       Impact factor: 3.441

5.  Nitric oxide causes the macroschizonts of Theileria annulata to disappear and host cells to become apoptotic.

Authors:  J O Richardson; L M Forsyth; C G Brown; P M Preston
Journal:  Vet Res Commun       Date:  1998-01       Impact factor: 2.459

6.  Macrophage migration inhibitory factor plays a critical role in mediating protection against the helminth parasite Taenia crassiceps.

Authors:  Miriam Rodríguez-Sosa; Lucia E Rosas; John R David; Rafael Bojalil; Abhay R Satoskar; Luis I Terrazas
Journal:  Infect Immun       Date:  2003-03       Impact factor: 3.441

7.  The unexpected role for the aryl hydrocarbon receptor on susceptibility to experimental toxoplasmosis.

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Journal:  J Biomed Biotechnol       Date:  2010-01-11

8.  The effects of dithiaden on nitric oxide production by RAW 264.7 cells.

Authors:  Jana Králová; Michaela Pekarová; Katarína Drábiková; Viera Jančinová; Radomír Nosáľ; Milan Cíž; Antonín Lojek
Journal:  Interdiscip Toxicol       Date:  2008-12

9.  Purification and partial biochemical characterization of a Mycoplasma fermentans-derived substance that activates macrophages to release nitric oxide, tumor necrosis factor, and interleukin-6.

Authors:  P F Mühlradt; M Frisch
Journal:  Infect Immun       Date:  1994-09       Impact factor: 3.441

10.  Chronic helminth infection induces alternatively activated macrophages expressing high levels of CCR5 with low interleukin-12 production and Th2-biasing ability.

Authors:  Miriam Rodríguez-Sosa; Abhay R Satoskar; Rodrigo Calderón; Lorena Gomez-Garcia; Rafael Saavedra; Rafael Bojalil; Luis I Terrazas
Journal:  Infect Immun       Date:  2002-07       Impact factor: 3.441
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