AIM: To investigate the potential involvement of apoptosis and its regulators Bcl-2, Bax, and Fas within the retina in Staphylococcus epidermidis experimental endophthalmitis. METHODS: Endophthalmitis was induced in 48 male Lewis rats by unilateral 25-mircrol intravitreal injection of 7,000 viable organisms of slime-producing S. epidermidis strain ATCC 35983 (experimental group). Forty-eight other Lewis rats received a similar sterile normal saline injection (control group). The injected eyes were graded for clinical inflammation and were removed in groups at 6, 12, 24, 48, 72, and 168 hours post-injection. After surgical separation, retinal tissue specimens were fixed, and paraffin sections underwent hematoxylin-eosin staining, immunohistochemistry against Bcl-2, Bax, and Fas, and TUNEL assay for detection of apoptotic cells. Following morphometric analysis, the apoptotic body index (ABI) was calculated. RESULTS: While Bcl-2 expression was absent, Bax and Fas expression and apoptosis in ganglion cells, bipolar cells, and photoreceptors, were significantly higher in the experimental group compared to the control group (P < 0.05). In the experimental group, inflammation peaked at 24 hours, Bax and Fas expression at 48 hours and the ABI at 72 hours post-injection. CONCLUSION: Apoptosis is increased within the retina in S. epidermidis experimental endophthalmitis through upregulation of Bax and Fas, peaking soon after peak inflammation.
AIM: To investigate the potential involvement of apoptosis and its regulators Bcl-2, Bax, and Fas within the retina in Staphylococcus epidermidis experimental endophthalmitis. METHODS:Endophthalmitis was induced in 48 male Lewis rats by unilateral 25-mircrol intravitreal injection of 7,000 viable organisms of slime-producing S. epidermidis strain ATCC 35983 (experimental group). Forty-eight other Lewis rats received a similar sterile normal saline injection (control group). The injected eyes were graded for clinical inflammation and were removed in groups at 6, 12, 24, 48, 72, and 168 hours post-injection. After surgical separation, retinal tissue specimens were fixed, and paraffin sections underwent hematoxylin-eosin staining, immunohistochemistry against Bcl-2, Bax, and Fas, and TUNEL assay for detection of apoptotic cells. Following morphometric analysis, the apoptotic body index (ABI) was calculated. RESULTS: While Bcl-2 expression was absent, Bax and Fas expression and apoptosis in ganglion cells, bipolar cells, and photoreceptors, were significantly higher in the experimental group compared to the control group (P < 0.05). In the experimental group, inflammation peaked at 24 hours, Bax and Fas expression at 48 hours and the ABI at 72 hours post-injection. CONCLUSION: Apoptosis is increased within the retina in S. epidermidis experimental endophthalmitis through upregulation of Bax and Fas, peaking soon after peak inflammation.
Authors: Ioannis K Petropoulos; Chrysoula V Vantzou; Fotini N Lamari; Nikolaos K Karamanos; Evangelos D Anastassiou; Nikolaos M Pharmakakis Journal: Graefes Arch Clin Exp Ophthalmol Date: 2006-03-17 Impact factor: 3.117
Authors: Emily A Whiston; Norito Sugi; Merideth C Kamradt; Coralynn Sack; Susan R Heimer; Michael Engelbert; Eric F Wawrousek; Michael S Gilmore; Bruce R Ksander; Meredith S Gregory Journal: Infect Immun Date: 2008-01-28 Impact factor: 3.441
Authors: Roger A Astley; Phillip S Coburn; Salai Madhumathi Parkunan; Michelle C Callegan Journal: Prog Retin Eye Res Date: 2016-05-03 Impact factor: 21.198
Authors: J L Vallejo-Garcia; M Asencio-Duran; N Pastora-Salvador; P Vinciguerra; M R Romano Journal: Mediators Inflamm Date: 2012-09-02 Impact factor: 4.711