| Literature DB >> 19031061 |
Radhika P Nagarkar1, Joel P Schneider.
Abstract
Hydrogels based on peptide self-assembly form an important class of biomaterials that find application in tissue engineering and drug delivery. It is essential to prepare peptides with high purity to achieve batch-to-batch consistency affording hydrogels with reproducible properties. Automated solid-phase peptide synthesis coupled with optimized Fmoc (9-fluorenylmethoxy-carbonyl) chemistry to obtain peptides in high yield and purity is discussed. Details of isolating a desired peptide from crude synthetic mixtures and assessment of the peptide's final purity by high-performance liquid chromatography and mass spectrometry are provided. Beyond the practical importance of synthesis and primary characterization, techniques used to investigate the properties of hydrogels are briefly discussed.Entities:
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Year: 2008 PMID： 19031061 PMCID： PMC2650242 DOI： 10.1007/978-1-59745-480-3_5
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745