| Literature DB >> 19019379 |
Dezso Csupor1, Eva Maria Wenzig, István Zupkó, Karin Wölkart, Judit Hohmann, Rudolf Bauer.
Abstract
By optimizing the extraction and analytical conditions, a reliable and precise HPLC method coupled with photodiode array detection (HPLC-DAD) has been developed for the identification and quantification of three major aconitine-type alkaloids (aconitine, mesaconitine, hypaconitine) in the roots of Aconitum carmichaelii Debeaux. The qualitative analysis of the plant material was carried out by LC-APCI-MS(n). By means of this method, 26 lipo-alkaloids were also identified from the roots of A. carmichaelii. The effect of processing on aconitine-type alkaloids, lipo-alkaloids and pure aconitine was studied. As part of our investigation, two lipo-alkaloids, 14-benzoylaconine-8-palmitate and 14-benzoylaconine-8-linoleate were produced semisynthetically. The COX-1, COX-2 and LTB(4) formation inhibitory activity of aconite root extracts and different types of diterpene alkaloids and the toxicity of lipo-alkaloids were also investigated.Entities:
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Year: 2008 PMID： 19019379 DOI： 10.1016/j.chroma.2008.10.082
Source DB: PubMed Journal: J Chromatogr A ISSN： 0021-9673 Impact factor: 4.759