María-Jesus Cruz1, Sara Sánchez-Vidaurre2, Pablo-Vicente Romero3, Ferran Morell2, Xavier Muñoz4. 1. Ciber Enfermedades Respiratorias, Fisiologia, Immunologia, Universidad Autònoma de Barcelona, Barcelona, Spain. Electronic address: mjcruz@ir.vhebron.net. 2. Servei de Pneumologia Hospital Vall d'Hebron, Fisiologia, Immunologia, Universidad Autònoma de Barcelona, Barcelona, Spain. 3. Universidad Autònoma de Barcelona; Servei de Pneumologia, Fisiologia, Immunologia, Universidad Autònoma de Barcelona, Barcelona, Spain. 4. Hospital de Bellvitge; and Departament de Biologia Cellular, Fisiologia, Immunologia, Universidad Autònoma de Barcelona, Barcelona, Spain.
Abstract
BACKGROUND: Few studies have addressed the effects of aging on levels of inflammatory markers in exhaled breath condensate (EBC). The aim of this study was to determine whether there are significant age-associated differences in pH, 8-isoprostane, and nitrogen oxide values in EBC from a population of healthy adults. MATERIAL AND METHODS: EBC samples were obtained from 75 healthy volunteers aged 18 to 80 years and stratified into five groups according to age (n = 15): 18 to 29, 30 to 39 years, 40 to 49 years, 50 to 59 years, and 60 to 80 years. The following were measured in the samples collected: pH before and after deaeration, nitrite, nitrate, and 8-isoprostane. Differences between the groups were assessed by the Kruskal-Wallis test. RESULTS: Significant differences in deaerated pH (p < 0.0001) were found in the group of individuals 60 to 80 years of age as compared to the remaining groups. Significant differences were also found in 8-isoprostane levels between the younger and older groups (18 to 29 years and 30 to 39 years of age; p = 0.006 and p = 0.034, respectively). There were no significant differences in nitrite or nitrate values between younger and older individuals. CONCLUSION: The results of this study indicate that pH and 8-isoprostane levels in EBC show a relationship with age. Thus, values obtained in studies with control groups may require adjustment for these factors.
BACKGROUND: Few studies have addressed the effects of aging on levels of inflammatory markers in exhaled breath condensate (EBC). The aim of this study was to determine whether there are significant age-associated differences in pH, 8-isoprostane, and nitrogen oxide values in EBC from a population of healthy adults. MATERIAL AND METHODS:EBC samples were obtained from 75 healthy volunteers aged 18 to 80 years and stratified into five groups according to age (n = 15): 18 to 29, 30 to 39 years, 40 to 49 years, 50 to 59 years, and 60 to 80 years. The following were measured in the samples collected: pH before and after deaeration, nitrite, nitrate, and 8-isoprostane. Differences between the groups were assessed by the Kruskal-Wallis test. RESULTS: Significant differences in deaerated pH (p < 0.0001) were found in the group of individuals 60 to 80 years of age as compared to the remaining groups. Significant differences were also found in 8-isoprostane levels between the younger and older groups (18 to 29 years and 30 to 39 years of age; p = 0.006 and p = 0.034, respectively). There were no significant differences in nitrite or nitrate values between younger and older individuals. CONCLUSION: The results of this study indicate that pH and 8-isoprostane levels in EBC show a relationship with age. Thus, values obtained in studies with control groups may require adjustment for these factors.
Authors: Anita Ljubičić Ćalušić; Veda Marija Varnai; Anka Ozana Cavlović; Maja Segvić Klarić; Ružica Beljo; Ljerka Prester; Jelena Macan Journal: Int Arch Occup Environ Health Date: 2012-09-25 Impact factor: 3.015
Authors: Rafael Fernandez; Gisela Gili; Ana Villagra; Josefina Lopez-Aguilar; Antonio Artigas Journal: Intensive Care Med Date: 2013-01-08 Impact factor: 17.440
Authors: Rembert Koczulla; Silvano Dragonieri; Robert Schot; Robert Bals; Stefanie A Gauw; Claus Vogelmeier; Klaus F Rabe; Peter J Sterk; Pieter S Hiemstra Journal: Respir Res Date: 2009-08-24
Authors: Thamires Marques de Lima; Cristiane Mayumi Kazama; Andreas Rembert Koczulla; Pieter S Hiemstra; Mariangela Macchione; Ana Luisa Godoy Fernandes; Ubiratan de Paula Santos; Maria Lucia Bueno-Garcia; Dirce Maria Zanetta; Carmen Diva Saldiva de André; Paulo Hilario Nascimento Saldiva; Naomi Kondo Nakagawa Journal: Clinics (Sao Paulo) Date: 2013-12 Impact factor: 2.365