Literature DB >> 19007188

Quantitative ESI-TOF analysis of macromolecular assembly kinetics.

Anne E Bunner1, Sunia A Trauger, Gary Siuzdak, James R Williamson.   

Abstract

The Escherichia coli small (30S) ribosomal subunit is a particularly well-characterized model system for studying in vitro self-assembly. A previously developed pulse-chase monitored by quantitative mass spectrometry (PC/QMS) approach to measuring kinetics of in vitro 30S assembly suffered from poor signal-to-noise and was unable to observe some ribosomal proteins. We have developed an improved LC-MS based method using quantitative ESI-TOF analysis of isotope-labeled tryptic peptides. Binding rates for 18 of the 20 ribosomal proteins are reported, and exchange of proteins S2 and S21 between bound and unbound states prevented measurement of their binding kinetics. Multiphasic kinetics of 3' domain proteins S7 and S9 are reported, which support an assembly mechanism that utilizes multiple parallel pathways. This quantitative ESI-TOF approach should be widely applicable to study the assembly of other macromolecular complexes and to quantitative proteomics experiments in general.

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Year:  2008        PMID： 19007188      PMCID： PMC2735594          DOI： 10.1021/ac8020505

Source DB:  PubMed          Journal:  Anal Chem        ISSN： 0003-2700            Impact factor:   6.986


  30 in total

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Journal:  J Mol Biol       Date:  1993-07-20       Impact factor: 5.469

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  14 in total

1.  Chemical modulators of ribosome biogenesis as biological probes.

Authors:  Jonathan M Stokes; Eric D Brown
Journal:  Nat Chem Biol       Date:  2015-11-17       Impact factor: 15.040

2.  Kinetic cooperativity in Escherichia coli 30S ribosomal subunit reconstitution reveals additional complexity in the assembly landscape.

Authors:  Anne E Bunner; Andrea H Beck; James R Williamson
Journal:  Proc Natl Acad Sci U S A       Date:  2010-03-05       Impact factor: 11.205

3.  Escherichia coli rimM and yjeQ null strains accumulate immature 30S subunits of similar structure and protein complement.

Authors:  Vivian Leong; Meredith Kent; Ahmad Jomaa; Joaquin Ortega
Journal:  RNA       Date:  2013-04-23       Impact factor: 4.942

4.  Quantitation of the ribosomal protein autoregulatory network using mass spectrometry.

Authors:  Michael T Sykes; Edit Sperling; Stephen S Chen; James R Williamson
Journal:  Anal Chem       Date:  2010-06-15       Impact factor: 6.986

5.  The effect of ribosome assembly cofactors on in vitro 30S subunit reconstitution.

Authors:  Anne E Bunner; Stefan Nord; P Mikael Wikström; James R Williamson
Journal:  J Mol Biol       Date:  2010-02-24       Impact factor: 5.469

6.  Quantitative proteomic analysis of ribosome assembly and turnover in vivo.

Authors:  Michael T Sykes; Zahra Shajani; Edit Sperling; Andrea H Beck; James R Williamson
Journal:  J Mol Biol       Date:  2010-08-13       Impact factor: 5.469

7.  Measuring the dynamics of E. coli ribosome biogenesis using pulse-labeling and quantitative mass spectrometry.

Authors:  Stephen S Chen; Edit Sperling; Josh M Silverman; Joseph H Davis; James R Williamson
Journal:  Mol Biosyst       Date:  2012-10-30

8.  Characterization of the ribosome biogenesis landscape in E. coli using quantitative mass spectrometry.

Authors:  Stephen S Chen; James R Williamson
Journal:  J Mol Biol       Date:  2012-12-07       Impact factor: 5.469

9.  A toolbox for ab initio 3-D reconstructions in single-particle electron microscopy.

Authors:  Neil R Voss; Dmitry Lyumkis; Anchi Cheng; Pick-Wei Lau; Anke Mulder; Gabriel C Lander; Edward J Brignole; Denis Fellmann; Christopher Irving; Erica L Jacovetty; Albert Leung; James Pulokas; Joel D Quispe; Hanspeter Winkler; Craig Yoshioka; Bridget Carragher; Clinton S Potter
Journal:  J Struct Biol       Date:  2009-12-16       Impact factor: 2.867

10.  Stable isotope pulse-chase monitored by quantitative mass spectrometry applied to E. coli 30S ribosome assembly kinetics.

Authors:  Anne E Bunner; James R Williamson
Journal:  Methods       Date:  2009-06-24       Impact factor: 3.608
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