| Literature DB >> 19003228 |
S Lenglois, M Moser, A O A Miller.
Abstract
CHO-K1 and VERO cells have been grown on MicroHextrade mark, a polystyrene-based microsupport with two-dimensional geometry and the consistency of their growth kinetics were established. These cells have been detached by exposure to buffered ethylenediaminetetraacetic acid (EDTA) followed by controlled mechanical shear to yield well-isolated cells suspended in EDTA. Neutralisation of the EDTA followed by restoration of the chemical composition of the growth medium causes detached CHO-K1 cells to display unaltered growth kinetics.Entities:
Year: 2004 PMID： 19003228 PMCID： PMC3449500 DOI： 10.1023/B:CYTO.0000043403.20008.02
Source DB: PubMed Journal: Cytotechnology ISSN： 0920-9069 Impact factor: 2.058