Literature DB >> 1900283

UGA can be decoded as tryptophan at low efficiency in Bacillus subtilis.

P S Lovett1, N P Ambulos, W Mulbry, N Noguchi, E J Rogers.   

Abstract

Replacement of cat-86 codon 7 or 144 with the UGA codon permitted the gene to confer chloramphenicol resistance in wild-type Bacillus subtilis. UAA replacements of the same codons resulted in a chloramphenicol-sensitive phenotype in wild-type B. subtilis and a chloramphenicol-resistant phenotype in suppressor-positive strains. N-terminal sequencing showed that UGA at codon 7 was decoded as tryptophan in wild-type cells, at an efficiency of about 6%.

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Year:  1991        PMID： 1900283      PMCID： PMC207334          DOI： 10.1128/jb.173.5.1810-1812.1991

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  15 in total

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