OBJECTIVE: To evaluated the transdentinal diffusion and subsequent cytotoxicity of self-etching adhesives on odontoblast-like cells. MATERIALS AND METHODS: Sixty dentin disks (0.4-mm thick) were produced from human molars and divided into six groups (n = 10). The dentin disks were placed in in vitro pulp chambers where MDPC-23 cells were planted on 0.28 cm(2) of exposed dentin on the pulpal side. The adhesives Clearfil SE Bond (CSE), Clearfil Protect Bond (CPB), Adper Prompt (PR), and Xeno III (XE) were applied on the occlusal side. Single Bond (SB) was used as positive and phosphate buffer solution (PBS) as negative control. The cytotoxicity was measured by MTT assay and cell characteristics were assessed by SEM. The transdentinal diffusion was qualified by GC/MS. RESULTS: Kruskal-Wallis and Mann-Whitney tests demonstrated a significant difference among the adhesives and PBS. Cellular viability reduction promoted by the self-etching systems was lower than that of SB (53.1%), except for CSE. Cell metabolism was reduced in 47.8%, 42.1%, 28.0%, and 46.5% for CSE, CPB, PR, and XE, respectively. HEMA was identified as the main diffused component. CONCLUSION: Components from all investigated self-etching adhesive systems were able to diffuse through the dentin resulting in significant reduction of the cellular metabolism.
OBJECTIVE: To evaluated the transdentinal diffusion and subsequent cytotoxicity of self-etching adhesives on odontoblast-like cells. MATERIALS AND METHODS: Sixty dentin disks (0.4-mm thick) were produced from human molars and divided into six groups (n = 10). The dentin disks were placed in in vitro pulp chambers where MDPC-23 cells were planted on 0.28 cm(2) of exposed dentin on the pulpal side. The adhesives Clearfil SE Bond (CSE), Clearfil Protect Bond (CPB), Adper Prompt (PR), and Xeno III (XE) were applied on the occlusal side. Single Bond (SB) was used as positive and phosphate buffer solution (PBS) as negative control. The cytotoxicity was measured by MTT assay and cell characteristics were assessed by SEM. The transdentinal diffusion was qualified by GC/MS. RESULTS: Kruskal-Wallis and Mann-Whitney tests demonstrated a significant difference among the adhesives and PBS. Cellular viability reduction promoted by the self-etching systems was lower than that of SB (53.1%), except for CSE. Cell metabolism was reduced in 47.8%, 42.1%, 28.0%, and 46.5% for CSE, CPB, PR, and XE, respectively. HEMA was identified as the main diffused component. CONCLUSION: Components from all investigated self-etching adhesive systems were able to diffuse through the dentin resulting in significant reduction of the cellular metabolism.
Authors: Ana Paula Silveira Turrioni; Camila Fávero de Oliveira; Fernanda Gonçalves Basso; Lilian Tan Moriyama; Cristina Kurachi; Josimeri Hebling; Vanderlei S Bagnato; Carlos Alberto de Souza Costa Journal: Lasers Med Sci Date: 2011-05-10 Impact factor: 3.161
Authors: Marília Oliveira Barbosa; Rodrigo Varella de Carvalho; Flávio Fernando Demarco; Fabrício Aulo Ogliari; Cesar Henrique Zanchi; Evandro Piva; Adriana Fernandes da Silva Journal: J Mater Sci Mater Med Date: 2015-01-15 Impact factor: 3.896
Authors: Débora Lopes Salles Scheffel; Nancy Tomoko Sacono; Ana Paula Dias Ribeiro; Diana Gabriela Soares; Fernanda Gonçalves Basso; David Pashley; Carlos Alberto de Souza Costa; Josimeri Hebling Journal: J Dent Date: 2015-03-06 Impact factor: 4.379
Authors: Luciana Bianchi; Ana Paula Dias Ribeiro; Marcela Rocha de Oliveira Carrilho; David H Pashley; Carlos Alberto de Souza Costa; Josimeri Hebling Journal: Dent Mater Date: 2013-07-29 Impact factor: 5.304