INTRODUCTION: This study was aimed to investigate the relationship between quantitative proton magnetic resonance spectroscopy (1H-MRS) and pathological changes in meningioma. MATERIALS AND METHODS: Twenty-two meningioma cases underwent single voxel 1H-MRS (point-resolved spectroscopy sequence, repetition time/echo time = 2,000 ms/68, 136, 272 ms). Absolute choline (Cho) concentration was calculated using tissue water as the internal reference and corrected according to intra-voxel cystic/necrotic parts. Pathological specimens were stained with MIB-1 antibody to measure cell density and proliferation index. Correlation analysis was performed between absolute Cho concentration and cell density and MIB-1 labeled proliferation index. RESULTS: Average Cho concentration of all meningiomas before correction was 2.95 +/- 0.86 mmol/kg wet weight. It was increased to 3.23 +/- 1.15 mmol/kg wet weight after correction. Average cell density of all meningiomas was 333 +/- 119 cells/HPF, and average proliferation index was 2.93 +/- 5.72%. A linear, positive correlation between cell density and Cho concentration was observed (r = 0.650, P = 0.001). After correction of Cho concentration, the correlation became more significant (r = 0.737, P < 0.001). However, no significant correlation between Cho concentration and proliferation index was found. There seemed to be a positive correlation trend after correction of Cho concentration but did not reach significant level. CONCLUSION: Absolute Cho concentration, especially Cho concentration corrected according to intra-voxel cystic/necrotic parts, reflects cell density of meningioma.
INTRODUCTION: This study was aimed to investigate the relationship between quantitative proton magnetic resonance spectroscopy (1H-MRS) and pathological changes in meningioma. MATERIALS AND METHODS: Twenty-two meningioma cases underwent single voxel 1H-MRS (point-resolved spectroscopy sequence, repetition time/echo time = 2,000 ms/68, 136, 272 ms). Absolute choline (Cho) concentration was calculated using tissue water as the internal reference and corrected according to intra-voxel cystic/necrotic parts. Pathological specimens were stained with MIB-1 antibody to measure cell density and proliferation index. Correlation analysis was performed between absolute Cho concentration and cell density and MIB-1 labeled proliferation index. RESULTS: Average Cho concentration of all meningiomas before correction was 2.95 +/- 0.86 mmol/kg wet weight. It was increased to 3.23 +/- 1.15 mmol/kg wet weight after correction. Average cell density of all meningiomas was 333 +/- 119 cells/HPF, and average proliferation index was 2.93 +/- 5.72%. A linear, positive correlation between cell density and Cho concentration was observed (r = 0.650, P = 0.001). After correction of Cho concentration, the correlation became more significant (r = 0.737, P < 0.001). However, no significant correlation between Cho concentration and proliferation index was found. There seemed to be a positive correlation trend after correction of Cho concentration but did not reach significant level. CONCLUSION: Absolute Cho concentration, especially Cho concentration corrected according to intra-voxel cystic/necrotic parts, reflects cell density of meningioma.
Authors: R K Gupta; T F Cloughesy; U Sinha; J Garakian; J Lazareff; G Rubino; L Rubino; D P Becker; H V Vinters; J R Alger Journal: J Neurooncol Date: 2000-12 Impact factor: 4.130
Authors: Sophia Dahmani; Nicholas Kaliss; John W VanMeter; David J Moore; Ronald J Ellis; Xiong Jiang Journal: Neurology Date: 2021-07-12 Impact factor: 11.800