BACKGROUND: Choline (Cho) containing compounds are usually evaluated using magnetic resonance spectroscopy (MRS) by relative ratios such as Cho/N-acetylaspartate (NAA) and Cho/creatine (Cre) ratios. To clarify the significance of Cho level in gliomas, we evaluated the quantified Cho level using MRS and compared it with the proliferation activity as determined by MIB-1 immunoreactivity in the histological specimen. METHODS: There were seven benign and seven malignant gliomas. MRS was obtained using a single-voxel proton regional imaging of metabolites (PRIME) sequence with three different TE for T2 compensation. Quantified Cho level was compared with the number of MIB-1 immunopositive positive cells and cell density in surgical specimens. RESULT: A positive correlation was observed between Cho and MIB-1 in benign gliomas, whereas there was a trend to an inverse correlation in malignant gliomas. This inverse correlation became a positive correlation when the necrotic area of the tumor (on the T1-weighted gadolinium enhanced images) was excluded from the voxel of interest (VOI) for MRS, but this correlation did not reach statistical significance. CONCLUSIONS: The quantification data clarified the behavior of Cho in malignant gliomas. The quantification method has the advantage of limiting the influence of other metabolites on Cho determination. In particular, the levels of other commonly measured metabolites, including Cre, may also be altered in glioma, making ratios between metabolites misleading. Heterogeneity in the MRS VOI should be considered when evaluating the proliferative activity of malignant glioma by MRS.
BACKGROUND:Choline (Cho) containing compounds are usually evaluated using magnetic resonance spectroscopy (MRS) by relative ratios such as Cho/N-acetylaspartate (NAA) and Cho/creatine (Cre) ratios. To clarify the significance of Cho level in gliomas, we evaluated the quantified Cho level using MRS and compared it with the proliferation activity as determined by MIB-1 immunoreactivity in the histological specimen. METHODS: There were seven benign and seven malignant gliomas. MRS was obtained using a single-voxel proton regional imaging of metabolites (PRIME) sequence with three different TE for T2 compensation. Quantified Cho level was compared with the number of MIB-1 immunopositive positive cells and cell density in surgical specimens. RESULT: A positive correlation was observed between Cho and MIB-1 in benign gliomas, whereas there was a trend to an inverse correlation in malignant gliomas. This inverse correlation became a positive correlation when the necrotic area of the tumor (on the T1-weighted gadolinium enhanced images) was excluded from the voxel of interest (VOI) for MRS, but this correlation did not reach statistical significance. CONCLUSIONS: The quantification data clarified the behavior of Cho in malignant gliomas. The quantification method has the advantage of limiting the influence of other metabolites on Cho determination. In particular, the levels of other commonly measured metabolites, including Cre, may also be altered in glioma, making ratios between metabolites misleading. Heterogeneity in the MRS VOI should be considered when evaluating the proliferative activity of malignant glioma by MRS.
Authors: James S Cordova; Hui-Kuo G Shu; Zhongxing Liang; Saumya S Gurbani; Lee A D Cooper; Chad A Holder; Jeffrey J Olson; Brad Kairdolf; Eduard Schreibmann; Stewart G Neill; Constantinos G Hadjipanayis; Hyunsuk Shim Journal: Neuro Oncol Date: 2016-03-15 Impact factor: 12.300
Authors: Patricia Coutinho de Souza; Krithika Balasubramanian; Charity Njoku; Natalyia Smith; David L Gillespie; Andrea Schwager; Osama Abdullah; Jerry W Ritchey; Kar-Ming Fung; Debra Saunders; Randy L Jensen; Rheal A Towner Journal: J Magn Reson Imaging Date: 2015-04-29 Impact factor: 4.813
Authors: Carlos E A Batista; Csaba Juhász; Otto Muzik; William J Kupsky; Geoffrey Barger; Harry T Chugani; Sandeep Mittal; Sandeep Sood; Pulak K Chakraborty; Diane C Chugani Journal: Mol Imaging Biol Date: 2009-05-12 Impact factor: 3.488
Authors: J Scott Cordova; Saumya S Gurbani; Jeffrey J Olson; Zhongxing Liang; Lee A D Cooper; Hui-Kuo G Shu; Eduard Schreibmann; Stewart G Neill; Constantinos G Hadjipanayis; Chad A Holder; Hyunsuk Shim Journal: Tomography Date: 2016-06