Literature DB >> 18996156

A simple method for the rapid removal of Bacillus anthracis spores from DNA preparations.

Leslie A Dauphin1, Michael D Bowen.   

Abstract

This study establishes a filtration method for the safe removal of Bacillus anthracis spores which may contaminate DNA preparations. Centrifugal filtration with 0.1-microm filter units can be used following extraction of DNA from B. anthracis spores to render samples safe without compromising the sensitivity of diagnostic real-time PCR assays for B. anthracis.

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Year:  2008        PMID： 18996156     DOI： 10.1016/j.mimet.2008.10.009

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  10 in total

1.  Verification of a Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Method for Diagnostic Identification of High-Consequence Bacterial Pathogens.

Authors:  Dobryan M Tracz; Kym S Antonation; Cindi R Corbett
Journal:  J Clin Microbiol       Date:  2015-12-16       Impact factor: 5.948

2.  Real-time polymerase chain reaction assay for rapid and sensitive detection of anthrax spores in spiked soil and talcum powder.

Authors:  Neha Jain; S Merwyn; G P Rai; G S Agarwal
Journal:  Folia Microbiol (Praha)       Date:  2012-04-17       Impact factor: 2.099

3.  Swab protocol for rapid laboratory diagnosis of cutaneous anthrax.

Authors:  Leslie A Dauphin; Chung K Marston; Vinod Bhullar; Daniel Baker; Mahmudur Rahman; M Jahangir Hossain; Apurba Chakraborty; Salah Uddin Khan; Alex R Hoffmaster
Journal:  J Clin Microbiol       Date:  2012-10-03       Impact factor: 5.948

4.  Evaluation of automated and manual commercial DNA extraction methods for recovery of Brucella DNA from suspensions and spiked swabs.

Authors:  Leslie A Dauphin; Rebecca J Hutchins; Liberty A Bost; Michael D Bowen
Journal:  J Clin Microbiol       Date:  2009-10-21       Impact factor: 5.948

5.  High stringency evaluation of the inactivation / exclusion efficacy of a MALDI-TOF MS chemical extraction method, with filtration of extract through 0.1 µm filters, on Bacillus anthracis Vollum vegetative cells and spores.

Authors:  Simon A Weller; Jennie Latham
Journal:  PLoS One       Date:  2017-05-08       Impact factor: 3.240

6.  Rapid identification of unknown pathogens in environmental samples using a high-throughput sequencing-based approach.

Authors:  Ofir Israeli; Inbar Cohen-Gihon; Anat Zvi; Shirley Lazar; Ohad Shifman; Haim Levy; Avital Tidhar; Adi Beth-Din
Journal:  Heliyon       Date:  2019-05-27

7.  Validated Methods for Removing Select Agent Samples from Biosafety Level 3 Laboratories.

Authors:  Alexandria E Kesterson; John E Craig; Lara J Chuvala; Henry S Heine
Journal:  Emerg Infect Dis       Date:  2020-11       Impact factor: 6.883

8.  Safety and Accuracy of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Highly Pathogenic Organisms.

Authors:  James T Rudrik; Marty K Soehnlen; Michael J Perry; Maureen M Sullivan; Wanda Reiter-Kintz; Philip A Lee; Denise Pettit; Anthony Tran; Erin Swaney
Journal:  J Clin Microbiol       Date:  2017-10-11       Impact factor: 5.948

9.  Genetic Diversity of Australian Bacillus anthracis Isolates Revealed by Multiple-Locus Variable-Number Tandem Repeat Analysis.

Authors:  Janine Muller; Ilhan Mohammad; Simone Warner; Roger Paskin; Fiona Constable; Mark Fegan
Journal:  Microorganisms       Date:  2020-06-11

Review 10.  Detection and Identification of Bacillus anthracis: From Conventional to Molecular Microbiology Methods.

Authors:  Aleksandra A Zasada
Journal:  Microorganisms       Date:  2020-01-16
  10 in total

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