Tohru Sakimoto1,2, Ai Yamada3, Hitoshi Kanno4, Mitsuru Sawa3. 1. Department of Ophthalmology, Nihon University School of Medicine, Tokyo, Japan. torusaki@med.nihon-u.ac.jp. 2. Department of Ophthalmology, Nihon University School of Medicine, 30-1 Oyaguchi Kamimachi, Itabashi-ku, Tokyo, 173-8610, Japan. torusaki@med.nihon-u.ac.jp. 3. Department of Ophthalmology, Nihon University School of Medicine, Tokyo, Japan. 4. Department of Transfusion Medicine and Cell Processing, Tokyo Women's Medical University, Tokyo, Japan.
Abstract
PURPOSE: To elucidate tumor necrosis factor (TNF) receptor-1 (TNFR1) and TNF-alpha converting enzyme (TACE) involvement during corneal wound healing. METHODS: The corneas of BALB/c mice cornea were scarred by alkali burns using filter paper dipped in 1N NaOH solution. TACE and TNFR1 expression in the alkali-burned corneas were evaluated by immunohistochemistry (total, 16 eyes). Using cultured fibroblasts (human) and macrophages (mice), we evaluated the release of soluble TNF-alpha (sTNF-alpha) and soluble TNFR1 (sTNFR1) in the supernatant by enzyme-linked immunosorbent assay after stimulating TACE activity with phorbol myristate acetate (PMA). RESULTS: In alkali-burned corneas, both TACE and TNFR1 expression were observed in the stromal cells after the acute phase of wound healing response. In macrophage-cultured supernatant, both sTNF-alpha and sTNFR1 release were promoted by PMA stimulation. On the other hand, only sTNFR1 released by PMA stimulation was observed in fibroblast-cultured supernatant. CONCLUSIONS: TACE and TNFR1 were expressed mainly after the acute phase of corneal wound healing. The TACE-dependent extracellular release of sTNFR1 was recognized in cultured fibroblasts and macrophages.
PURPOSE: To elucidate tumor necrosis factor (TNF) receptor-1 (TNFR1) and TNF-alpha converting enzyme (TACE) involvement during corneal wound healing. METHODS: The corneas of BALB/c mice cornea were scarred by alkali burns using filter paper dipped in 1N NaOH solution. TACE and TNFR1 expression in the alkali-burned corneas were evaluated by immunohistochemistry (total, 16 eyes). Using cultured fibroblasts (human) and macrophages (mice), we evaluated the release of soluble TNF-alpha (sTNF-alpha) and soluble TNFR1 (sTNFR1) in the supernatant by enzyme-linked immunosorbent assay after stimulating TACE activity with phorbol myristate acetate (PMA). RESULTS: In alkali-burned corneas, both TACE and TNFR1 expression were observed in the stromal cells after the acute phase of wound healing response. In macrophage-cultured supernatant, both sTNF-alpha and sTNFR1 release were promoted by PMA stimulation. On the other hand, only sTNFR1 released by PMA stimulation was observed in fibroblast-cultured supernatant. CONCLUSIONS:TACE and TNFR1 were expressed mainly after the acute phase of corneal wound healing. The TACE-dependent extracellular release of sTNFR1 was recognized in cultured fibroblasts and macrophages.
Authors: M F McDermott; I Aksentijevich; J Galon; E M McDermott; B W Ogunkolade; M Centola; E Mansfield; M Gadina; L Karenko; T Pettersson; J McCarthy; D M Frucht; M Aringer; Y Torosyan; A M Teppo; M Wilson; H M Karaarslan; Y Wan; I Todd; G Wood; R Schlimgen; T R Kumarajeewa; S M Cooper; J P Vella; C I Amos; J Mulley; K A Quane; M G Molloy; A Ranki; R J Powell; G A Hitman; J J O'Shea; D L Kastner Journal: Cell Date: 1999-04-02 Impact factor: 41.582
Authors: David S Smookler; Fazilat F Mohammed; Zamaneh Kassiri; Gordon S Duncan; Tak W Mak; Rama Khokha Journal: J Immunol Date: 2006-01-15 Impact factor: 5.422
Authors: M L Moss; S L Jin; M E Milla; D M Bickett; W Burkhart; H L Carter; W J Chen; W C Clay; J R Didsbury; D Hassler; C R Hoffman; T A Kost; M H Lambert; M A Leesnitzer; P McCauley; G McGeehan; J Mitchell; M Moyer; G Pahel; W Rocque; L K Overton; F Schoenen; T Seaton; J L Su; J D Becherer Journal: Nature Date: 1997-02-20 Impact factor: 49.962
Authors: P Reddy; J L Slack; R Davis; D P Cerretti; C J Kozlosky; R A Blanton; D Shows; J J Peschon; R A Black Journal: J Biol Chem Date: 2000-05-12 Impact factor: 5.157
Authors: J J Peschon; J L Slack; P Reddy; K L Stocking; S W Sunnarborg; D C Lee; W E Russell; B J Castner; R S Johnson; J N Fitzner; R W Boyce; N Nelson; C J Kozlosky; M F Wolfson; C T Rauch; D P Cerretti; R J Paxton; C J March; R A Black Journal: Science Date: 1998-11-13 Impact factor: 47.728
Authors: Francesco Pichi; Paola Carrai; Sunil K Srivastava; Careen Y Lowder; Paolo Nucci; Piergiorgio Neri Journal: Int Ophthalmol Date: 2015-10-09 Impact factor: 2.031
Authors: Andrea DeSantis-Rodrigues; Yoke-Chen Chang; Rita A Hahn; Iris P Po; Peihong Zhou; C Jeffrey Lacey; Abhilash Pillai; Sherri C Young; Robert A Flowers; Michael A Gallo; Jeffrey D Laskin; Donald R Gerecke; Kathy K H Svoboda; Ned D Heindel; Marion K Gordon Journal: Invest Ophthalmol Vis Sci Date: 2016-04 Impact factor: 4.799