Literature DB >> 18988016

Designing experiments for high-throughput protein expression.

Stephen P Chambers1, Susanne E Swalley.   

Abstract

The advent of high-throughput protein production and the vast amount of data it is capable of generating has created both new opportunities and problems. Automation and miniaturization allow experimentation to be performed more efficiently, justifying the cost involved in establishing a high-throughput platform. These changes have also magnified the need for effective statistical methods to identify trends and relationships in the data. The application of quantitative management tools to this process provides the means of ensuring maximum efficiency and productivity.

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Year:  2009        PMID: 18988016     DOI: 10.1007/978-1-59745-196-3_2

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  10 in total

1.  Optimization of Soluble Expression and Purification of Recombinant Human Rhinovirus Type-14 3C Protease Using Statistically Designed Experiments: Isolation and Characterization of the Enzyme.

Authors:  Georgia Antoniou; Irineos Papakyriacou; Christos Papaneophytou
Journal:  Mol Biotechnol       Date:  2017-10       Impact factor: 2.695

Review 2.  Antibody-based protein multiplex platforms: technical and operational challenges.

Authors:  Allison A Ellington; Iftikhar J Kullo; Kent R Bailey; George G Klee
Journal:  Clin Chem       Date:  2009-12-03       Impact factor: 8.327

3.  A general path for large-scale solubilization of cellular proteins: from membrane receptors to multiprotein complexes.

Authors:  Filippo Pullara; Jennifer Guerrero-Santoro; Monica Calero; Qiangmin Zhang; Ye Peng; Henrik Spåhr; Guy L Kornberg; Antonella Cusimano; Hilary P Stevenson; Hugo Santamaria-Suarez; Shelley L Reynolds; Ian S Brown; Satdarshan P S Monga; Bennett Van Houten; Vesna Rapić-Otrin; Guillermo Calero; Arthur S Levine
Journal:  Protein Expr Purif       Date:  2012-11-05       Impact factor: 1.650

4.  Optimization of production of the anti-keratin 8 single-chain Fv TS1-218 in Pichia pastoris using design of experiments.

Authors:  Rozbeh Jafari; Birgitta E Sundström; Patrik Holm
Journal:  Microb Cell Fact       Date:  2011-05-16       Impact factor: 5.328

5.  Automated small-scale protein purification and analysis for accelerated development of protein therapeutics.

Authors:  Xavier LeSaout; Matteo Costioli; Lynn Jordan; Jeremy Lambert; Ross Beighley; Laurel Provencher; Brian Gerwe; Kevin McGuire; Nico Verlinden; Andrew Barry
Journal:  Eng Life Sci       Date:  2015-10-07       Impact factor: 2.678

6.  Optimization of Recombinant Expression of Synthetic Bacterial Phytase in Pichia pastoris Using Response Surface Methodology.

Authors:  Ali Akbarzadeh; Ehsan Dehnavi; Mojtaba Aghaeepoor; Jafar Amani
Journal:  Jundishapur J Microbiol       Date:  2015-12-26       Impact factor: 0.747

7.  Statistically Designed Medium Reveals Interactions between Metabolism and Genetic Information Processing for Production of Stable Human Serum Albumin in Pichia pastoris.

Authors:  Nitu Maity; Saroj Mishra
Journal:  Biomolecules       Date:  2019-10-04

Review 8.  Recombinant antibody production evolves into multiple options aimed at yielding reagents suitable for application-specific needs.

Authors:  Ario de Marco
Journal:  Microb Cell Fact       Date:  2015-09-02       Impact factor: 5.328

9.  Exploring codon optimization and response surface methodology to express biologically active transmembrane RANKL in E. coli.

Authors:  Sushila Maharjan; Bijay Singh; Jin-Duck Bok; Jeong-In Kim; Tao Jiang; Chong-Su Cho; Sang-Kee Kang; Yun-Jaie Choi
Journal:  PLoS One       Date:  2014-05-08       Impact factor: 3.240

10.  Preparation and Antitumor Activity of CS5931, A Novel Polypeptide from Sea Squirt Ciona Savignyi.

Authors:  Xiaoshuang Chen; Huanli Xu; Bo Li; Feng Wang; Xiaoliang Chen; Dexin Kong; Xiukun Lin
Journal:  Mar Drugs       Date:  2016-03-21       Impact factor: 5.118

  10 in total

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