Literature DB >> 18979017

Microinjection of A. aegypti embryos to obtain transgenic mosquitoes.

Nijole Jasinskiene1, Jennifer Juhn, Anthony A James.   

Abstract

In this video, Nijole Jasinskiene demonstrates the methodology employed to generate transgenic Aedes aegypti mosquitoes, which are vectors for dengue fever. The techniques for correctly preparing microinjection needles, desiccating embryos, and performing microinjection are demonstrated.

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Year:  2007        PMID： 18979017      PMCID： PMC2557089          DOI： 10.3791/219

Source DB:  PubMed          Journal:  J Vis Exp        ISSN： 1940-087X            Impact factor:   1.355


  21 in total

1.  Transgene-mediated suppression of dengue viruses in the salivary glands of the yellow fever mosquito, Aedes aegypti.

Authors:  G Mathur; I Sanchez-Vargas; D Alvarez; K E Olson; O Marinotti; A A James
Journal:  Insect Mol Biol       Date:  2010-12       Impact factor: 3.585

Review 2.  How to turn an organism into a model organism in 10 'easy' steps.

Authors:  Benjamin J Matthews; Leslie B Vosshall
Journal:  J Exp Biol       Date:  2020-02-07       Impact factor: 3.312

3.  Delivery of Nucleic Acids through Embryo Microinjection in the Worldwide Agricultural Pest Insect, Ceratitis capitata.

Authors:  Paolo Gabrieli; Francesca Scolari
Journal:  J Vis Exp       Date:  2016-10-01       Impact factor: 1.355

4.  Aedes aegypti: an emerging model for vector mosquito development.

Authors:  Anthony Clemons; Morgan Haugen; Ellen Flannery; Michael Tomchaney; Kristopher Kast; Caitlin Jacowski; Christy Le; Akio Mori; Wendy Simanton Holland; Joseph Sarro; David W Severson; Molly Duman-Scheel
Journal:  Cold Spring Harb Protoc       Date:  2010-10-01

5.  Functional analysis of genes in Aedes aegypti embryos.

Authors:  Anthony Clemons; Morgan Haugen; David Severson; Molly Duman-Scheel
Journal:  Cold Spring Harb Protoc       Date:  2010-10-01

6.  Comparison of transgene expression in Aedes aegypti generated by mariner Mos1 transposition and ΦC31 site-directed recombination.

Authors:  Alexander W E Franz; N Jasinskiene; I Sanchez-Vargas; A T Isaacs; M R Smith; C C H Khoo; M S Heersink; A A James; K E Olson
Journal:  Insect Mol Biol       Date:  2011-06-24       Impact factor: 3.585

7.  Collagen-binding protein, Aegyptin, regulates probing time and blood feeding success in the dengue vector mosquito, Aedes aegypti.

Authors:  Andrezza Campos Chagas; José Luis Ramirez; Nijole Jasinskiene; Anthony A James; José M C Ribeiro; Osvaldo Marinotti; Eric Calvo
Journal:  Proc Natl Acad Sci U S A       Date:  2014-04-28       Impact factor: 11.205

8.  Targeted genome editing in Aedes aegypti using TALENs.

Authors:  Azadeh Aryan; Kevin M Myles; Zach N Adelman
Journal:  Methods       Date:  2014-02-18       Impact factor: 3.608

9.  The RNA interference pathway affects midgut infection- and escape barriers for Sindbis virus in Aedes aegypti.

Authors:  Cynthia C H Khoo; Joseph Piper; Irma Sanchez-Vargas; Ken E Olson; Alexander W E Franz
Journal:  BMC Microbiol       Date:  2010-04-28       Impact factor: 3.605

10.  Methods for TALEN Evaluation, Use, and Mutation Detection in the Mosquito Aedes aegypti.

Authors:  Sanjay Basu; Azadeh Aryan; Mary Etna Haac; Kevin M Myles; Zach N Adelman
Journal:  Methods Mol Biol       Date:  2016
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