| Literature DB >> 18977349 |
Qingli Niu1, Jianxun Luo, Guiquan Guan, Zhijie Liu, Miling Ma, Aihong Liu, Jinliang Gao, Qiaoyun Ren, Youquan Li, Jiaxiang Qiu, H Yin.
Abstract
The first and second internal transcribed spacers (ITS1, ITS2) as well as the intervening 5.8S coding region of the rRNA gene for six Babesia spp. isolated from different geographic origins were characterized. Varying degrees of ITS1 and ITS2 intra- and inter-species sequence polymorphism were found among these isolates. Phylogenetic analysis of the ITS1-5.8S gene-ITS2 region clearly separated the isolates into two clusters. One held an unidentified Babesia sp. transmitted by Hyalomma anatolicum anatolicum. The second held five other isolates, which were considered to be Babesia motasi. Each Babesia species cluster possessed ITS1 and ITS2 of unique size(s) and species specific nucleotide sequences. The results showed that ITS1, ITS2 and the complete ITS1-5.8S-ITS2 region could be used to discriminate these ovine Babesia spp. effectively.Entities:
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Year: 2008 PMID： 18977349 DOI： 10.1016/j.exppara.2008.09.021
Source DB: PubMed Journal: Exp Parasitol ISSN： 0014-4894 Impact factor: 2.011