Literature DB >> 1895887

Synthesis of 6-phosphatidyl-L-ascorbic acid by phospholipase D.

A Nagao1, N Ishida, J Terao.   

Abstract

Phospholipase D (EC 3.1.4.4) of Streptomyces species was found to catalyze transphosphatidylation to L-ascorbic acid from phosphatidylcholine (PC) in a biphasic reaction system. The product was identified as 1,2-diacyl-sn-glycero-3-phospho-6'-L-ascorbic acid (PA-AsA) by mass spectrometry and nuclear magnetic resonance spectroscopy. The optimal pH of transphosphatidylation was 4.5 and the rate of PA-AsA formation increased as concentrations of L-ascorbic acid increased. The conversion of PC to PA-AsA was greater than 80%. PA-AsA was found to be more resistant to hydrolysis by phospholipase D than was PC.

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Year:  1991        PMID： 1895887     DOI： 10.1007/bf02537205

Source DB:  PubMed          Journal:  Lipids        ISSN： 0024-4201            Impact factor:   1.880


  18 in total

1.  Antioxidant activity of 6-phosphatidyl-L-ascorbic acid.

Authors:  A Nagao; J Terao
Journal:  Biochem Biophys Res Commun       Date:  1990-10-30       Impact factor: 3.575

2.  Conversion of radiolabelled ethanolamine plasmalogen into the dimethylethanolamine and choline analogue via transphosphatidylation by phospholipase D from cabbage.

Authors:  V Achterberg; H Fricke; G Gercken
Journal:  Chem Phys Lipids       Date:  1986 Oct-Nov       Impact factor: 3.329

3.  A facile enzymatic synthesis of 5'-(3-sn-phosphatidyl)nucleosides and their antileukemic activities.

Authors:  S Shuto; H Itoh; S Ueda; S Imamura; K Fukukawa; M Tsujino; A Matsuda; T Ueda
Journal:  Chem Pharm Bull (Tokyo)       Date:  1988-01       Impact factor: 1.645

4.  Synthetic I-acylglycerophospholipid analogues as lipid substrates. I. Studies on microsomal acylation and uptake into liver slices of I-acyl analogues containing various polar bases.

Authors:  P F Jezyk; H N Hughes
Journal:  Biochim Biophys Acta       Date:  1973-01-19

Review 5.  Antioxidants in relation to lipid peroxidation.

Authors:  E Niki
Journal:  Chem Phys Lipids       Date:  1987 Jul-Sep       Impact factor: 3.329

6.  Transphosphatidylation by phospholipase D.

Authors:  S F Yang; S Freer; A A Benson
Journal:  J Biol Chem       Date:  1967-02-10       Impact factor: 5.157

7.  Stabilization of phosphatidylethanolamine hydroperoxides in human plasma.

Authors:  J Terao
Journal:  Biochim Biophys Acta       Date:  1989-06-08

8.  Liposome oxidation and erythrocyte lysis by enzymically generated superoxide and hydrogen peroxide.

Authors:  E W Kellogg; I Fridovich
Journal:  J Biol Chem       Date:  1977-10-10       Impact factor: 5.157

9.  The enzymatic synthesis of phosphatidylserine and purification by CM-cellulose column chromatography.

Authors:  P Comfurius; R F Zwaal
Journal:  Biochim Biophys Acta       Date:  1977-07-20

10.  Kinetic evaluation of conversion of phosphatidylcholine to phosphatidylethanolamine by phospholipase D from different sources.

Authors:  L R Juneja; T Kazuoka; T Yamane; S Shimizu
Journal:  Biochim Biophys Acta       Date:  1988-06-15
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  2 in total

1.  Synthesis of a phosphatidyl derivative of vitamin E and its antioxidant activity in phospholipid bilayers.

Authors:  T Koga; A Nagao; J Terao; K Sawada; K Mukai
Journal:  Lipids       Date:  1994-02       Impact factor: 1.880

2.  Purification, biochemical characterization, and cloning of phospholipase D from Streptomyces racemochromogenes strain 10-3.

Authors:  Yozo Nakazawa; Yoshimasa Sagane; Teppei Kikuchi; Masataka Uchino; Takeshi Nagai; Hiroaki Sato; Kazuki Toeda; Katsumi Takano
Journal:  Protein J       Date:  2010-11       Impact factor: 2.371
  2 in total

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