Literature DB >> 18931446

Effect of leucine-to-methionine substitutions on the diffraction quality of histone chaperone SET/TAF-Ibeta/INHAT crystals.

Miki Senda1, Shinsuke Muto, Masami Horikoshi, Toshiya Senda.   

Abstract

One of the most frequent problems in crystallization is poor quality of the crystals. In order to overcome this obstacle several methods have been utilized, including amino-acid substitutions of the target protein. Here, an example is presented of crystal-quality improvement by leucine-to-methionine substitutions. A variant protein with three amino-acid substitutions enabled improvement of the crystal quality of the histone chaperone SET/TAF-Ibeta/INHAT when combined with optimization of the cryoconditions. This procedure improved the resolution of the SET/TAF-Ibeta/INHAT crystals from around 5.5 to 2.3 A without changing the crystallization conditions.

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Year:  2008        PMID： 18931446      PMCID： PMC2564889          DOI： 10.1107/S1744309108028704

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  22 in total

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4.  Relationship between the structure of SET/TAF-Ibeta/INHAT and its histone chaperone activity.

Authors:  Shinsuke Muto; Miki Senda; Yusuke Akai; Lui Sato; Toru Suzuki; Ryozo Nagai; Toshiya Senda; Masami Horikoshi
Journal:  Proc Natl Acad Sci U S A       Date:  2007-03-06       Impact factor: 11.205

5.  Solving the structure of human H ferritin by genetically engineering intermolecular crystal contacts.

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8.  The impact of Glu-->Ala and Glu-->Asp mutations on the crystallization properties of RhoGDI: the structure of RhoGDI at 1.3 A resolution.

Authors:  Agnieszka Mateja; Yancho Devedjiev; Daniel Krowarsch; Kenton Longenecker; Zbigniew Dauter; Jacek Otlewski; Zygmunt S Derewenda
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9.  Crystal engineering yields crystals of cyclophilin D diffracting to 1.7 A resolution.

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10.  Flash-freezing causes a stress-induced modulation in a crystal structure of soybean lipoxygenase l3.

Authors:  E Skrzypczak-Jankun; M A Bianchet; L Mario Amzel; M O Funk
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