Literature DB >> 18931124

Altered oligomerization properties of N316 mutants of Escherichia coli TyrR.

Takashi Koyanagi1, Takane Katayama, Hideyuki Suzuki, Hidehiko Kumagai.   

Abstract

The transcriptional regulator TyrR is known to undergo a dimer-to-hexamer conformational change in response to aromatic amino acids, through which it controls gene expression. In this study, we identified N316D as the second-site suppressor of Escherichia coli TyrR(E274Q), a mutant protein deficient in hexamer formation. N316 variants exhibited altered in vivo regulatory properties, and the most drastic changes were observed for TyrR(N316D) and TyrR(N316R) mutants. Gel filtration analyses revealed that the ligand-mediated oligomer formation was enhanced and diminished for TyrR(N316D) and TyrR(N316R), respectively, compared with the wild-type TyrR. ADP was substituted for ATP in the oligomer formation of TyrR(N316D).

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Year:  2008        PMID： 18931124      PMCID： PMC2593242          DOI： 10.1128/JB.00889-08

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  29 in total

1.  Transcriptional regulation of tyrosine phenol-lyase gene mediated through TyrR and cAMP receptor protein.

Authors:  T Katayama; H Suzuki; K Yamamoto; H Kumagai
Journal:  Biosci Biotechnol Biochem       Date:  1999-10       Impact factor: 2.043

2.  Indirect readout of DNA sequence at the primary-kink site in the CAP-DNA complex: DNA binding specificity based on energetics of DNA kinking.

Authors:  S Chen; J Vojtechovsky; G N Parkinson; R H Ebright; H M Berman
Journal:  J Mol Biol       Date:  2001-11-16       Impact factor: 5.469

3.  Solution structure of the DNA-binding domain of the TyrR protein of Haemophilus influenzae.

Authors:  Y Wang; S Zhao; R L Somerville; O Jardetzky
Journal:  Protein Sci       Date:  2001-03       Impact factor: 6.725

4.  The influence of ATP on the association and unfolding of the tyrosine repressor ligand response domain of Haemophilus influenzae.

Authors:  S Kristl; S Zhao; S F Falsone; R L Somerville; A J Kungl
Journal:  Biochem Biophys Res Commun       Date:  2001-01-12       Impact factor: 3.575

5.  Functional analysis of the Erwinia herbicola tutB gene and its product.

Authors:  Takane Katayama; Hideyuki Suzuki; Takashi Koyanagi; Hidehiko Kumagai
Journal:  J Bacteriol       Date:  2002-06       Impact factor: 3.490

6.  folA, a new member of the TyrR regulon in Escherichia coli K-12.

Authors:  Ji Yang; Yoshito Ogawa; Helen Camakaris; Tomohiro Shimada; Akira Ishihama; A J Pittard
Journal:  J Bacteriol       Date:  2007-06-08       Impact factor: 3.490

7.  Cloning and random mutagenesis of the Erwinia herbicola tyrR gene for high-level expression of tyrosine phenol-lyase.

Authors:  T Katayama; H Suzuki; T Koyanagi; H Kumagai
Journal:  Appl Environ Microbiol       Date:  2000-11       Impact factor: 4.792

8.  The influence of ATP on the binding of aromatic amino acids to the ligand response domain of the tyrosine repressor of Haemophilus influenzae.

Authors:  S Kristl; S Zhao; B Knappe; R L Somerville; A J Kungl
Journal:  FEBS Lett       Date:  2000-02-04       Impact factor: 4.124

9.  Backbone resonance assignment of the 2H, 13C, 15N labelled 32kDa central domain of Escherichia coli TyrR.

Authors:  James D Swarbrick; Tanya Bashtannyk; Mathew Dixon; Richard N Pau; Barrie E Davidson; Paul R Gooley
Journal:  J Biomol NMR       Date:  2002-04       Impact factor: 2.835

10.  The central domain of Escherichia coli TyrR is responsible for hexamerization associated with tyrosine-mediated repression of gene expression.

Authors:  Mathew P Dixon; Richard N Pau; Geoffrey J Howlett; David E Dunstan; William H Sawyer; Barrie E Davidson
Journal:  J Biol Chem       Date:  2002-03-28       Impact factor: 5.157
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  1 in total

Review 1.  Microbial production of isoquinoline alkaloids as plant secondary metabolites based on metabolic engineering research.

Authors:  Fumihiko Sato; Hidehiko Kumagai
Journal:  Proc Jpn Acad Ser B Phys Biol Sci       Date:  2013       Impact factor: 3.493
  1 in total

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