Literature DB >> 1892954

Isolation of restrictible DNA.

E Topić1, J Gluhak.   

Abstract

A simple method for the isolation of pure and high-yield DNA from whole blood, suitable for restriction enzyme digestion, is described. The steps of the procedure are as follows: cell lysis with NH4Cl, NaHCO3, EDTA; digestion with proteinase K in the presence of SDS; extraction with phenol-chloroform-isoamyl alcohol; and precipitation with ethanol. The 260 nm/280 nm absorbance ratio showed a mean value of 2, and the average yield of DNA was 212 micrograms/l. Such DNA preparations were found to be quite suitable for digestion by a variety of restriction endonucleases, as well as for the analysis of gene disorders by different biological methods. The method proposed appears to be useful in clinical chemistry laboratories.

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Year:  1991        PMID： 1892954

Source DB:  PubMed          Journal:  Eur J Clin Chem Clin Biochem        ISSN： 0939-4974


  6 in total

1.  SLC46A1 Haplotype with Predicted Functional Impact has Prognostic Value in Breast Carcinoma.

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Journal:  Mol Diagn Ther       Date:  2021-01-02       Impact factor: 4.074

2.  Evidence of a correlation between mannose binding lectin and celiac disease: a model for other autoimmune diseases.

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Journal:  J Mol Med (Berl)       Date:  2005-01-06       Impact factor: 4.599

3.  Single Nucleotide Variants in KIF14 Gene May Have Prognostic Value in Breast Cancer.

Authors:  Ivona Krus; Veronika Brynychová; Viktor Hlaváč; Radka Václavíková; Maria Kováčová; Renata Koževnikovová; Katerina Kopečková; Jannis Tornikidis; David Vrána; Jiří Gatěk; Pavel Souček
Journal:  Mol Diagn Ther       Date:  2022-10-03       Impact factor: 4.476

4.  Targeted Sequencing of Pancreatic Adenocarcinomas from Patients with Metachronous Pulmonary Metastases.

Authors:  Viktor Hlavac; Beatrice Mohelnikova-Duchonova; Martin Lovecek; Jiri Ehrmann; Veronika Brynychova; Katerina Kolarova; Pavel Soucek
Journal:  Genes (Basel)       Date:  2020-11-24       Impact factor: 4.096

5.  Whole-exome sequencing of epithelial ovarian carcinomas differing in resistance to platinum therapy.

Authors:  Viktor Hlaváč; Petr Holý; Radka Václavíková; Lukáš Rob; Martin Hruda; Marcela Mrhalová; Petr Černaj; Jiří Bouda; Pavel Souček
Journal:  Life Sci Alliance       Date:  2022-10-13

6.  Non-coding polymorphisms in nucleotide binding domain 1 in ABCC1 gene associate with transcript level and survival of patients with breast cancer.

Authors:  Tereza Kunická; Radka Václavíková; Viktor Hlaváč; David Vrána; Václav Pecha; Karel Rauš; Markéta Trnková; Kateřina Kubáčková; Miloslav Ambruš; Ludmila Vodičková; Pavel Vodička; Pavel Souček
Journal:  PLoS One       Date:  2014-07-31       Impact factor: 3.240
  6 in total

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