Literature DB >> 1892399

Rapid and sensitive assay for the phytotoxin rhizobitoxine.

X Ruan1, N K Peters.   

Abstract

Rhizobitoxine is a phytotoxin synthesized by some strains of the legume symbiont genus Bradyrhizobium and the plant pathogen Pseudomonas andropogonis. We demonstrate here a new enzymatic assay which is 100-fold more sensitive than previous assays and can detect as little as 1.0 pmol of rhizobitoxine. The assay is based on the inhibition of Salmonella typhimurium beta-cystathionase by rhizobitoxine. Interestingly, beta-cystathionase from Bradyrhizobium japonicum is insensitive to rhizobitoxine at concentrations lower than 75 microM.

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Year:  1991        PMID： 1892399      PMCID： PMC183529          DOI： 10.1128/aem.57.7.2097-2100.1991

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  7 in total

1.  Rhizobial-Induced Chlorosis in Soybeans: Isolation, Production in Nodules, and Varietal Specificity of the Toxin.

Authors:  L D Owens; D A Wright
Journal:  Plant Physiol       Date:  1965-09       Impact factor: 8.340

2.  Production of the Soybean-Chlorosis Toxin by Rhizobium japonicum in Pure Culture.

Authors:  L D Owens; D A Wright
Journal:  Plant Physiol       Date:  1965-09       Impact factor: 8.340

3.  Symbiotic effectiveness of indigenous soybean bradyrhizobia as related to serological, morphological, rhizobitoxine, and hydrogenase phenotypes.

Authors:  J Fuhrmann
Journal:  Appl Environ Microbiol       Date:  1990-01       Impact factor: 4.792

4.  Rhizobium-synthesized phytototoxin: an inhibitor of beta-cystathionase in Salmonella typhimurium.

Authors:  L D Owens; S Guggenheim; J L Hilton
Journal:  Biochim Biophys Acta       Date:  1968-05

5.  Cystathionine gamma-synthetase of Salmonella. Structural properties of a new enzyme in bacterial methionine biosynthesis.

Authors:  M M Kaplan; M Flavin
Journal:  J Biol Chem       Date:  1966-12-25       Impact factor: 5.157

6.  Mechanism of inhibition of spinach beta-cystathionase by rhizobitoxine.

Authors:  J Giovanelli; L D Owens; S H Mudd
Journal:  Biochim Biophys Acta       Date:  1971-03-10

7.  Nodule formation is stimulated by the ethylene inhibitor aminoethoxyvinylglycine.

Authors:  N K Peters; D K Crist-Estes
Journal:  Plant Physiol       Date:  1989-10       Impact factor: 8.340
  7 in total
  6 in total

1.  DNA sequence and mutational analysis of rhizobitoxine biosynthesis genes in Bradyrhizobium elkanii.

Authors:  T Yasuta; S Okazaki; H Mitsui; K Yuhashi; H Ezura; K Minamisawa
Journal:  Appl Environ Microbiol       Date:  2001-11       Impact factor: 4.792

2.  Foliar Chlorosis in Symbiotic Host and Nonhost Plants Induced by Rhizobium tropici Type B Strains.

Authors:  K P O'connell; J Handelsman
Journal:  Appl Environ Microbiol       Date:  1993-07       Impact factor: 4.792

3.  Bradyrhizobium japonicum rhizobitoxine genes and putative enzyme functions: expression requires a translational frameshift.

Authors:  X Ruan; C Zhang; N K Peters
Journal:  Proc Natl Acad Sci U S A       Date:  1993-04-01       Impact factor: 11.205

4.  Bradyrhizobium elkanii rtxC gene is required for expression of symbiotic phenotypes in the final step of rhizobitoxine biosynthesis.

Authors:  Shin Okazaki; Masayuki Sugawara; Kiwamu Minamisawa
Journal:  Appl Environ Microbiol       Date:  2004-01       Impact factor: 4.792

5.  New assay for rhizobitoxine based on inhibition of 1-aminocyclopropane-1-carboxylate synthase.

Authors:  T Yasuta; S Satoh; K Minamisawa
Journal:  Appl Environ Microbiol       Date:  1999-02       Impact factor: 4.792

6.  Isolation and characterization of rhizobitoxine mutants of Bradyrhizobium japonicum.

Authors:  X Ruan; N K Peters
Journal:  J Bacteriol       Date:  1992-06       Impact factor: 3.490
  6 in total

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