Literature DB >> 1892261

Enzyme-linked immunosorbent assay for the detection of Salmonella enteritidis infection in chickens.

C J Kim1, K V Nagaraja, B S Pomeroy.   

Abstract

An ELISA was developed and tested for its ability to detect antibodies against Salmonella enteritidis in chickens. Various features of the ELISA were evaluated and optimized. The outer membrane protein antigens selected by use of the protein immunoblotting method made the assay specific and sensitive. The assay was evaluated in chickens experimentally infected with S enteritidis. Blood samples collected at weekly intervals after experimental infection with S enteritidis were analyzed by ELISA. Results of the ELISA were compared with those of conventional serum plate and microagglutination tests. The ELISA was more sensitive and specific in the detection of S enteritidis infection than the other 2 conventional tests.

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Year:  1991        PMID： 1892261

Source DB:  PubMed          Journal:  Am J Vet Res        ISSN： 0002-9645            Impact factor:   1.156


  7 in total

Review 1.  ELISAs and the serological analysis of Salmonella infections in poultry: a review.

Authors:  P A Barrow
Journal:  Epidemiol Infect       Date:  1992-12       Impact factor: 2.451

2.  Immune response induced by ppGpp-defective Salmonella enterica serovar Gallinarum in chickens.

Authors:  Sang-Ik Park; Jae-Ho Jeong; Hyon E Choy; Joon Haeng Rhee; Hee-Sam Na; Tae-Hoon Lee; Moon Her; Kyoung-Oh Cho; Yeongjin Hong
Journal:  J Microbiol       Date:  2010-11-03       Impact factor: 3.422

3.  World Health Organisation--supervised interlaboratory comparison of ELISAs for the serological detection of Salmonella enterica serotype Enteritidis in chickens.

Authors:  P A Barrow; M Desmidt; R Ducatelle; M Guittet; H M van der Heijden; P S Holt; J H Huis in't Velt; P McDonough; K V Nagaraja; R E Porter; K Proux; F Sisak; C Staak; G Steinbach; C J Thorns; C Wray; F van Zijderveld
Journal:  Epidemiol Infect       Date:  1996-08       Impact factor: 2.451

4.  Development and application of enzyme-linked immunosorbent assay for specific detection of Salmonella enteritidis infections in chickens based on antibodies to SEF14 fimbrial antigen.

Authors:  C J Thorns; M M Bell; M G Sojka; R A Nicholas
Journal:  J Clin Microbiol       Date:  1996-04       Impact factor: 5.948

5.  Application of an indirect immunofluorescent staining method for detection of Salmonella enteritidis in paraffin slices and antigen location in infected duck tissues.

Authors:  Bin Yan; An-Chun Cheng; Ming-Shu Wang; Shu-Xuan Deng; Zhen-Hua Zhang; Nian-Chun Yin; Ping Cao; Sheng-Yan Cao
Journal:  World J Gastroenterol       Date:  2008-02-07       Impact factor: 5.742

6.  Comparison of four different enzyme-linked immunosorbent assays for serological diagnosis of Salmonella enteritidis infections in experimentally infected chickens.

Authors:  F G van Zijderveld; A M van Zijderveld-van Bemmel; J Anakotta
Journal:  J Clin Microbiol       Date:  1992-10       Impact factor: 5.948

7.  Interpretations of antibody responses to Salmonella enterica serotype enteritidis gm flagellin in poultry flocks are enhanced by a kinetics-based enzyme-linked immunosorbent assay.

Authors:  P L McDonough; R H Jacobson; J F Timoney; A Mutalib; D C Kradel; Y F Chang; S J Shin; D H Lein; S Trock; K Wheeler
Journal:  Clin Diagn Lab Immunol       Date:  1998-07
  7 in total

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