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Literature DB >> 18922071

Increased detectability of plasma HIV-1 RNA after introduction of a new assay and altered specimen-processing procedures.

Peter F Rebeiro¹, Asghar Kheshti, Sally S Bebawy, Samuel E Stinnette, Husamettin Erdem, Yi-Wei Tang, Timothy R Sterling, Stephen P Raffanti, Richard T D'Aquila.

Abstract

After changes to assay and specimen-processing methods, plasma human immunodeficiency virus type 1 (HIV-1) RNA was frequently detectable in patients who previously had well-suppressed HIV-1 RNA levels. This artifact is attributable to shipping frozen plasma in primary plasma preparation tubes and is not caused by the HIV-1 RNA detection assay; it can be avoided by shipping plasma in a secondary tube.

Entities: Chemical Disease Gene Species

Mesh：

Substances：
RNA, Viral

Year: 2008 PMID： 18922071 PMCID： PMC2605467 DOI： 10.1086/592693

Source DB: PubMed Journal: Clin Infect Dis ISSN： 1058-4838 Impact factor: 9.079

10 in total

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4. Full suppression of viral load is needed to achieve an optimal CD4 cell count response among patients on triple drug antiretroviral therapy.

Authors: E Wood; B Yip; R S Hogg; C H Sherlock; N Jahnke; R P Harrigan; M V O'Shaughnessy; J S Montaner
Journal: AIDS Date: 2000-09-08 Impact factor: 4.177

5. Revised recommendations for HIV screening of pregnant women.

Authors:
Journal: MMWR Recomm Rep Date: 2001-11-09

6. Plasma viral load and CD4+ lymphocytes as prognostic markers of HIV-1 infection.

Authors: J W Mellors; A Muñoz; J V Giorgi; J B Margolick; C J Tassoni; P Gupta; L A Kingsley; J A Todd; A J Saah; R Detels; J P Phair; C R Rinaldo
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Review 7. Solid organ transplantation: referral, management, and outcomes in HIV-infected patients.

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8. Discordance between viral loads determined by Roche COBAS AMPLICOR human immunodeficiency virus type 1 monitor (version 1.5) Standard and ultrasensitive assays caused by freezing patient plasma in centrifuged becton-dickinson vacutainer brand plasma preparation tubes.

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9. Transient viremia in HIV-infected patients and use of plasma preparation tubes.

Authors: Valentina Stosor; Frank J Palella; Baiba Berzins; Michele Till; Angel Leake; Joan S Chmiel; Robert L Murphy
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10. Elevation of viral load by PCR and use of plasma preparation tubes for quantification of human immunodeficiency virus type 1.

Authors: S García-Bujalance; C Ladrón de Guevara; J González-García; J R Arribas; F Zamora; A Gutiérrez
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10 in total

9 in total

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2. Comparative performances of HIV-1 RNA load assays at low viral load levels: results of an international collaboration.

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3. Coamplification of HIV-1 proviral DNA and viral RNA in assays used for quantification of HIV-1 RNA.

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4. Successful use of Plasma Preparation Tubes™ (PPTs) in the COBAS® AmpliPrep/COBAS® TaqMan® HIV-1 test.

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5. Overestimation of human immunodeficiency virus type 1 load caused by the presence of cells in plasma from plasma preparation tubes.

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