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Literature DB >> 18854594

A guide to the perplexed on the specificity of antibodies.

Abstract

Many investigators are unaware of the potential problems with specificity of antibodies and the need to document antibody characterization meticulously for each antibody that is used. In this review, I consider the principles of antibody action and how they define a set of rules for what information should be obtained by the investigator before using an antibody in a serious scientific investigation.

Mesh：

Substances：
Antibodies
Antigens

Year: 2008 PMID： 18854594 PMCID： PMC2605712 DOI： 10.1369/jhc.2008.952770

Source DB: PubMed Journal: J Histochem Cytochem ISSN： 0022-1554 Impact factor: 2.479

6 in total

1. Fluorescent antibody methods.

Authors: A H COONS
Journal: Gen Cytochem Methods Date: 1958

2. Microwave oven-based technique for immunofluorescent staining of paraffin-embedded tissues.

Authors: Delwin J Long; Colleen Buggs
Journal: J Mol Histol Date: 2007-07-25 Impact factor: 2.611

3. Antigen retrieval with protease digestion applied in immunohistochemical diagnosis of Alport syndrome.

Authors: Na Guan; Li-Xia Yu; Guo-Hong Wu; Yan Xing; Jie Ding
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Review 4. Formaldehyde fixation.

Authors: C H Fox; F B Johnson; J Whiting; P P Roller
Journal: J Histochem Cytochem Date: 1985-08 Impact factor: 2.479

5. Specificity of immunoreactions: the importance of testing specificity in each method.

Authors: Andrea Lorincz; Zoltan Nusser
Journal: J Neurosci Date: 2008-09-10 Impact factor: 6.167

Review 6. Antibody diversification by somatic mutation: from Burnet onwards.

Authors: Michael S Neuberger
Journal: Immunol Cell Biol Date: 2008-01-08 Impact factor: 5.126

6 in total

60 in total

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3. Immunocytochemical localization of TASK-3 protein (K2P9.1) in the rat brain.

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Review 4. An introduction to proteoglycans and their localization.

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Review 5. Fixation strategies for retinal immunohistochemistry.

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6. Non-specific immunostaining by a rabbit antibody against gustducin α subunit in mouse brain.

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7. Recombinant renewable polyclonal antibodies.

Authors: Fortunato Ferrara; Sara D'Angelo; Tiziano Gaiotto; Leslie Naranjo; Hongzhao Tian; Susanne Gräslund; Elena Dobrovetsky; Peter Hraber; Fridtjof Lund-Johansen; Silvia Saragozza; Daniele Sblattero; Csaba Kiss; Andrew R M Bradbury
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