BACKGROUND: Renoprotection of liver-type fatty acid binding protein (L-FABP) was demonstrated in a streptozotocin (STZ)-induced diabetic mouse model. METHODS: Established human L-FABP (hL-FABP) transgenic (Tg) mice and wild-type (WT) mice were divided into two groups: diabetic mice were uninephrectomized and injected with STZ; control mice were uninephrectomized and injected with a citrate buffer alone. Although mouse L-FABP was not expressed in WT mice, hL-FABP was expressed in the proximal tubules of the diabetic Tg mice and in the control Tg mice at 8 and 14 weeks after these injections. RESULTS: The expression of renal hL-FABP increased significantly in diabetic Tg mice compared to control Tg mice. A number of macrophages (F4/80) infiltrating the interstitium, the gene expressions of MCP-1, MCP-3, TGF-beta, Fas, Bax and RAGE were significantly lower in diabetic Tg kidneys compared with diabetic WT kidneys. In the diabetic Tg kidneys, the degree of the tubulointerstitial injury and the deposition of type IV collagen were significantly lower than that of diabetic WT kidneys. The expressions of catalase and glutathione peroxidase-1 were significantly lower in diabetic Tg kidneys compared with diabetic WT kidneys. CONCLUSIONS: Renal L-FABP ameliorated the tubulointerstitial damage of type 1 diabetic mice.
BACKGROUND: Renoprotection of liver-type fatty acid binding protein (L-FABP) was demonstrated in a streptozotocin (STZ)-induced diabeticmouse model. METHODS: Established humanL-FABP (hL-FABP) transgenic (Tg) mice and wild-type (WT) mice were divided into two groups: diabeticmice were uninephrectomized and injected with STZ; control mice were uninephrectomized and injected with a citrate buffer alone. Although mouseL-FABP was not expressed in WT mice, hL-FABP was expressed in the proximal tubules of the diabetic Tg mice and in the control Tg mice at 8 and 14 weeks after these injections. RESULTS: The expression of renal hL-FABP increased significantly in diabetic Tg mice compared to control Tg mice. A number of macrophages (F4/80) infiltrating the interstitium, the gene expressions of MCP-1, MCP-3, TGF-beta, Fas, Bax and RAGE were significantly lower in diabetic Tg kidneys compared with diabetic WT kidneys. In the diabetic Tg kidneys, the degree of the tubulointerstitial injury and the deposition of type IV collagen were significantly lower than that of diabetic WT kidneys. The expressions of catalase and glutathione peroxidase-1 were significantly lower in diabetic Tg kidneys compared with diabetic WT kidneys. CONCLUSIONS: Renal L-FABP ameliorated the tubulointerstitial damage of type 1 diabeticmice.
Authors: Heather A Hostetler; Madhumitha Balanarasimha; Huan Huang; Matthew S Kelzer; Alagammai Kaliappan; Ann B Kier; Friedhelm Schroeder Journal: J Lipid Res Date: 2010-07-13 Impact factor: 5.922
Authors: Heather A Hostetler; Avery L McIntosh; Barbara P Atshaves; Stephen M Storey; H Ross Payne; Ann B Kier; Friedhelm Schroeder Journal: J Lipid Res Date: 2009-03-16 Impact factor: 5.922
Authors: Ivana Ivanišević; Amira Peco-Antić; Irena Vuličević; Dorđe Hercog; Vladimir Milovanović; Jelena Kotur-Stevuljević; Aleksandra Stefanović; Nikola Kocev Journal: Pediatr Nephrol Date: 2013-02-14 Impact factor: 3.714
Authors: Gregory G Martin; Barbara P Atshaves; Huan Huang; Avery L McIntosh; Brad J Williams; Pei-Jing Pai; David H Russell; Ann B Kier; Friedhelm Schroeder Journal: Am J Physiol Gastrointest Liver Physiol Date: 2009-10-08 Impact factor: 4.052