| Literature DB >> 18848527 |
Meagan Roddy1, Timothy P Fox, Jonathan P McFadden, Kenta Nakamura, Katia Del Rio-Tsonis, Panagiotis A Tsonis.
Abstract
To examine underlying mechanisms of urodele lens regeneration we have employed a proteomic analysis of 650 proteins involved in several signaling pathways. We compared expression of these proteins between the regeneration-competent dorsal iris and the regeneration-incompetent ventral iris in the newt. After a series of screenings we selected several proteins to evaluate their expression quantitatively on immunoblots. We then used these selected proteins to compare their expression between the dorsal iris of the newt and the iris of the axolotl, another urodele, which does not regenerate the lens. In the newt we find that most proteins are expressed in both dorsal and ventral iris, even though there is differential regulation. Moreover, several of these proteins are expressed in the axolotl iris as well and for some of them their expression is consistent with the regeneration potential.Entities:
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Year: 2008 PMID： 18848527 PMCID： PMC2597088 DOI： 10.1016/j.bbrc.2008.09.144
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575