BACKGROUND: The present study was designed to assess the effect of sex steroids (testosterone and 17beta-estradiol) on insulin receptor expression, insulin binding and glucose oxidation in human liver cell line. METHODS: Non-malignant Chang liver cells were treated with different concentrations of testosterone and 17beta-estradiol dissolved in serum free medium for 24 h to identify the effective dose of both steroids for further studies. Cells with 70-80% confluency were challenged with testosterone (0.1 micromol/l), 17beta-estradiol (0.1 micromol/l) and their combination along with insulin as a positive control for 24 h. After the treatment period, insulin receptor mRNA expression, cell surface insulin binding and (14)C-glucose oxidation were assessed. RESULTS: Both testosterone and 17beta-estradiol significantly increased the insulin receptor mRNA expression, cell surface insulin binding and (14)C-glucose oxidation compared to basal, but the increase was not at par with the effect of insulin. Compared to individual effects of testosterone and 17beta-estradiol, their combination significantly increased the glucose oxidation similar to that of insulin. CONCLUSION: It is concluded from the present study that testosterone and 17beta-estradiol can directly enhance insulin receptor mRNA expression, insulin binding and glucose oxidation in Chang liver cells and thereby glucose metabolism.
BACKGROUND: The present study was designed to assess the effect of sex steroids (testosterone and 17beta-estradiol) on insulin receptor expression, insulin binding and glucose oxidation in human liver cell line. METHODS: Non-malignant Chang liver cells were treated with different concentrations of testosterone and 17beta-estradiol dissolved in serum free medium for 24 h to identify the effective dose of both steroids for further studies. Cells with 70-80% confluency were challenged with testosterone (0.1 micromol/l), 17beta-estradiol (0.1 micromol/l) and their combination along with insulin as a positive control for 24 h. After the treatment period, insulin receptor mRNA expression, cell surface insulin binding and (14)C-glucose oxidation were assessed. RESULTS: Both testosterone and 17beta-estradiol significantly increased the insulin receptor mRNA expression, cell surface insulin binding and (14)C-glucose oxidation compared to basal, but the increase was not at par with the effect of insulin. Compared to individual effects of testosterone and 17beta-estradiol, their combination significantly increased the glucose oxidation similar to that of insulin. CONCLUSION: It is concluded from the present study that testosterone and 17beta-estradiol can directly enhance insulin receptor mRNA expression, insulin binding and glucose oxidation in Chang liver cells and thereby glucose metabolism.
Authors: Grace Huang; Karol M Pencina; Zhuoying Li; Shehzad Basaria; Shalender Bhasin; Thomas G Travison; Thomas W Storer; S Mitchell Harman; Panayiotis Tsitouras Journal: J Clin Endocrinol Metab Date: 2018-04-01 Impact factor: 5.958
Authors: Biljana Musicki; Anthony J Bella; Trinity J Bivalacqua; Kelvin P Davies; Michael E DiSanto; Nestor F Gonzalez-Cadavid; Johanna L Hannan; Noel N Kim; Carol A Podlasek; Christopher J Wingard; Arthur L Burnett Journal: J Sex Med Date: 2015-12-08 Impact factor: 3.802
Authors: Grace Huang; Karol Pencina; Zhuoying Li; Caroline M Apovian; Thomas G Travison; Thomas W Storer; Thiago Gagliano-Jucá; Shehzad Basaria; Shalender Bhasin Journal: J Gerontol A Biol Sci Med Sci Date: 2021-05-22 Impact factor: 6.053
Authors: Paulina Kur; Agnieszka Kolasa-Wołosiuk; Kamila Misiakiewicz-Has; Barbara Wiszniewska Journal: Int J Environ Res Public Health Date: 2020-04-11 Impact factor: 3.390
Authors: Nataliya V Yaglova; Dibakhan A Tsomartova; Sergey S Obernikhin; Valentin V Yaglov; Svetlana V Nazimova; Elina S Tsomartova; Elizaveta V Chereshneva; Marina Y Ivanova; Tatiana A Lomanovskaya Journal: Int J Mol Sci Date: 2021-03-19 Impact factor: 5.923