Literature DB >> 18828627

A comparative study of electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) versus SCX-IMAC-based methods for phosphopeptide isolation/enrichment.

Chee Sian Gan1, Tiannan Guo, Huoming Zhang, Sai Kiang Lim, Siu Kwan Sze.   

Abstract

Electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) has been introduced recently for phosphopeptide enrichment. Here we compared ERLIC with the well-established SCX-IMAC for identifying phosphopeptides in EGF-treated A431 cells. The ERLIC approach detected a higher number of phosphopeptides (17 311) than SCX-IMAC (4850), but it only detected 926 unique phosphopeptides compared to 1315 in SCX-IMAC. Only 12% unique phosphopeptides were common to both approaches, suggesting that more comprehensive phosphoproteomes could be generated by complementing SCX-IMAC with ERLIC.

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Year:  2008        PMID： 18828627     DOI： 10.1021/pr800473j

Source DB:  PubMed          Journal:  J Proteome Res        ISSN： 1535-3893            Impact factor:   4.466


  20 in total

1.  Global molecular dysfunctions in gastric cancer revealed by an integrated analysis of the phosphoproteome and transcriptome.

Authors:  Tiannan Guo; Sze Sing Lee; Wai Har Ng; Yi Zhu; Chee Sian Gan; Jiang Zhu; Haixia Wang; Shiang Huang; Siu Kwan Sze; Oi Lian Kon
Journal:  Cell Mol Life Sci       Date:  2010-10-16       Impact factor: 9.261

Review 2.  The coming of age of phosphoproteomics--from large data sets to inference of protein functions.

Authors:  Philippe P Roux; Pierre Thibault
Journal:  Mol Cell Proteomics       Date:  2013-09-13       Impact factor: 5.911

3.  Simultaneous characterization of glyco- and phosphoproteomes of mouse brain membrane proteome with electrostatic repulsion hydrophilic interaction chromatography.

Authors:  Huoming Zhang; Tiannan Guo; Xin Li; Arnab Datta; Jung Eun Park; Jie Yang; Sai Kiang Lim; James P Tam; Siu Kwan Sze
Journal:  Mol Cell Proteomics       Date:  2010-01-04       Impact factor: 5.911

4.  Online nanoflow multidimensional fractionation for high efficiency phosphopeptide analysis.

Authors:  Scott B Ficarro; Yi Zhang; Marlene J Carrasco-Alfonso; Brijesh Garg; Guillaume Adelmant; James T Webber; C John Luckey; Jarrod A Marto
Journal:  Mol Cell Proteomics       Date:  2011-07-25       Impact factor: 5.911

5.  Detection, evaluation and minimization of nonenzymatic deamidation in proteomic sample preparation.

Authors:  Piliang Hao; Yan Ren; Andrew J Alpert; Siu Kwan Sze
Journal:  Mol Cell Proteomics       Date:  2011-07-22       Impact factor: 5.911

Review 6.  Quantitative proteomic analysis of histone modifications.

Authors:  He Huang; Shu Lin; Benjamin A Garcia; Yingming Zhao
Journal:  Chem Rev       Date:  2015-02-17       Impact factor: 60.622

7.  Sequential enrichment with titania-coated magnetic mesoporous hollow silica microspheres and zirconium arsenate-modified magnetic nanoparticles for the study of phosphoproteome of HL60 cells.

Authors:  Qiong-Wei Yu; Xiao-Shui Li; Yongsheng Xiao; Lei Guo; Fan Zhang; Qian Cai; Yu-Qi Feng; Bi-Feng Yuan; Yinsheng Wang
Journal:  J Chromatogr A       Date:  2014-09-11       Impact factor: 4.759

8.  Lysine methylation of progesterone receptor at activation function 1 regulates both ligand-independent activity and ligand sensitivity of the receptor.

Authors:  Hwa Hwa Chung; Siu Kwan Sze; Amanda Rui En Woo; Yang Sun; Kae Hwan Sim; Xue Ming Dong; Valerie C-L Lin
Journal:  J Biol Chem       Date:  2014-01-10       Impact factor: 5.157

9.  Epithelial wounds induce differential phosphorylation changes in response to purinergic and EGF receptor activation.

Authors:  Amanuel Kehasse; Celeste B Rich; Albert Lee; Mark E McComb; Catherine E Costello; Vickery Trinkaus-Randall
Journal:  Am J Pathol       Date:  2013-10-01       Impact factor: 4.307

10.  Peptide orientation affects selectivity in ion-exchange chromatography.

Authors:  Andrew J Alpert; Konstantinos Petritis; Lars Kangas; Richard D Smith; Karl Mechtler; Goran Mitulović; Shabaz Mohammed; Albert J R Heck
Journal:  Anal Chem       Date:  2010-06-15       Impact factor: 6.986
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